Integral University 2010 M.Tech Biotechnology Fermentation technology - Question Paper
Roll No.
9
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SECOND SEMESTER EXAMINATION, 2009-10 FERMENTATION TECHNOLOGY
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Time 3 Hours Total Marks : 100
Note : (i) Attempt any Five questions.
(ii) Marks are indicated against each question.
(iii) Use of calculator is permitted.
O (iv) Refer to r| Vs <f> and Plots.
1. (a) Derive an expression for continuous ferrnontor when production is directly linked with energy metabolism. 10
(b) A strain of Escherichia coli has been genetically engineered to produce human protein. A batch culture is started by inoculating 12g cells into a 100 litre bubble column fermenter containing 10g/l glucose. The maximum specific growth rate of the culture is 0.9h_1; the biomass yield from glucose is 0.575gg\ 10
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(i) Estimate the Urns required to reach stationary phase.
(ii) What will be final cell density if the fermental ion is stopped
after only 70% of the substrete is consumed?
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2. (a) Derive ;he substrate concentration profile in a spherical bead for
rirsi and zero order Kinetics. 10
(b) r-Mhy hamster kidney cells are immobilised in alginate beads. The average particle diameter is 5mm. Rateaof oxygen consumption at bulk concentration of 8 x 10~3 Kg 02 rrf3 is 8.4 x 10 Kg s1 m~3 catalyst. The effective diffusivity of oxygen in the beads is
1.88 x 10~9 rrf s1, Assume that the'oxygen concentration at the surface of the catalyst is equal to the bulk concentration, and that
oxygen uptake follows zero order kinotio: 10
(i) internal moss transt't r effect:.. significant? p
ii} Wrist reaction rate would bo observed if diffusiona! _ resistance were eliminates?
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3. ,n; cuss the Kinetics of batch culture for substrate utilisation.
product formation and biomass generation. 10
(b) In y -ed batch -culture operating with intermittent addition of glucose solution, values of the following parameters are given at
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time t = 2h, when the system is at quasi steady state, v = 1000ml, (Fiow rate), F = 200ml/h, S0 = lOOg glucose/l, .Mm h~1,t
Ks = 0.1 g glucose/l, Yx/S = 0.5gg" cells/g glucose, X0 = 30g.
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(i) Find V0 (the initial volume of the culture). ,
(ii) Determine the concentration of growth limiting sjbstrate in ;:- vessel at quasi-steady state.
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(iii) Determine the concentration and total amount of biomass in the vessel at t = 2h (at quasi-steady state).
4. (a) What do you understand by solid state fermentation? Discuss the*-** factors which effect solid state fermentation. 10
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(b) A solution polymerization is to be carried out to 95% conversion in a series of stirred tank reactors, all operating at the same
temperature. Batch tests show that the reaction is first order to * monomer, and 95% conversion is reached in 6 hours. 10 Q
, If four reactors of ogj.v- s:zc ; ,ioial uic-time is needed?
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(ii) What fraction of the total heat released is generated in each*"
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(a) Comment on medium formulation. Discuss the major comporr..
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of fermentation media. 1\:
(b) Discuss the industrial production of ethanol. Comment on ti :. factors which affect the production of ethanol. i,
(a) What do you mean by scale up9 Discuss the physico' biological parameters of scale up in detail. 10
(b) Let us have a bubble column with an H/D ratio of 3, diameter 0.5m and a gas flow rate of 0.1 m3 h-1, which gives a superfici3-gas velocity of 0.25m s1. What.would be the liquid media fio-. rate? .10
OR
Explain the principle and working of commonly used sensors ; bioprosessing industry. T'
Write short notes on any Four: 20
(a) Biosensors
(b) L-Lysine production.
(c) Rules of thumb for scale up
(d) Fed batch culture
(e) Effectiveness factor (internal and external)
(f) On line controllers 0
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(g) Scale down
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Attachment: |
| Earning: Approval pending. |
