Acharya Nagarjuna University (ANU) 2007 Post Graduate Diploma PLANT AND ANIMAL TISSUE CULTURE - Question Paper

(DBT 03)

P.G.DIPLOMA EXAMINATION, MAY 2007.

Biotechnology

Paper III – PLANT AND ANIMAL TISSUE CULTURE

AND GENETIC ENGINEERING

Time: 3 hours Maximum: 100 Marks

ans any 5 ques.

All ques. carry equal marks.

1. What is a callus ? discuss the initiation and maintenance of callus.

2. discuss the procedure of single cell culture. provide its uses.

3. explain the methods of protoplast isolation and its culture.

4. What is a haploid plant ? explain the methods of its production.

5. provide the biochemical basis for every of the constituents of tissue culture medium.

6. explain the basic techniques and kinds of mammalian cell cultures.

7. Write the definition of every of the subsequent items giving their importance in tissue culture.
(a) Micro carrier culture (b) Cell synchronization
(c) Cell growth (d) Cell transformation.

8. Write an explanatory essay on stem cell culture and its applications.

9. Write an essay on gene therapy

10. provide an account of the methods of identification and expression of cloned genes.

Earning:   Approval pending.