Acharya Nagarjuna University (ANU) 2005 M.Sc Microbiology II - MICROBIOLOGICAL METHODS - Question Paper

M.Sc. (Previous) DEGREE EXAMINATION, DECEMBER 2005
First Year
Microbiology
Paper II - MICROBIOLOGICAL METHODS

Time : 3 hours Maximum : 100 marks

PART A - (5 X eight = 40 marks)
ans any 5 of the subsequent.
All ques. carry equal marks.

1. Phase contrast microscopy.
2. Use of hot air oven.
3. Pour plate method.
4. Winogradsky column.
5. Plaque assay.
6. HPLC.
7. SDS - PAGE.
8. Beer - Lambert legal regulations.

PART B (4 x 15 = 60 marks)
ans all the 4 ques..
UNIT I

9. (a) Write the principles and importance of scanning and transmission electron microscopy.

or

(b) define the composition and properties of general bacterial media.

UNIT II

10. (a) Write an account on various anaerobic culturing techniques.

or

(b) discuss the methods for maintenance and preservation of microbial cultures.

UNIT III

11. (a) explain the principles and applications of paper chromatography.

or

(b) Write a general account on various centrifugational techniques.

UNIT IV

12. (a) define the principles and applications of UV - VIS spectrophotometry.

or

(b) discuss the use of radio active substances in biology.

Earning:   Approval pending.