M.Sc-M.Sc Botany 2nd Year Paper - VIII : PLANT BIOTECHNOLOGY (Acharya Nagarjuna University - Center for Distance Education, Guntur, Andhra Pradesh-2013)
(DBOT 24)
M.Sc. (Final) DEGREE EXAMINATION, MAY - 2013
Second Year
Botany
Paper - VIII : PLANT BIOTECHNOLOGY
Time : 03 Hours Maximum Marks : 100
Section - A (5 × 8 = 40)
Answer any Five of the following
1) Micropropagation.
2) Culture medium.
3) Synthetic seeds.
4) Somatic hybridization.
5) Gene manipulation.
6) PCR.
7) Biolistic method of gene transfer.
8) PVC vector. Section - B (4 × 15 = 60)
Answer All of the following 9) a) Describe the methods of androgenic haploid production and their uses.
OR
b) Explain the method of in vitro selection of mutants.
10) a) What is biotransformation? Explain with the help of suitable examples.
OR
b) What is somatic hybridization? Describe the various techniques used for protoplast
fusion. 11) a) Explain the technique of Western blotting and its uses.
OR
b) Distinguish between genomic and cDNA libraries. Explain the method of construction
of cDNA library.
12) a) Write an essay on the application of gene transformation techniques in crop
improvement.
OR
b) Distinguish between RAPD and RFLP. Describe the use of RAPDs in gene mapping.
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Earning: Approval pending. |