M.Sc-M.Sc Botany 2nd Year Paper - VIII : PLANT BIOTECHNOLOGY (Acharya Nagarjuna University - Center for Distance Education, Guntur, Andhra Pradesh-2013)

(DBOT 24) 
M.Sc. (Final) DEGREE EXAMINATION, MAY - 2013 
Second Year 
Botany 
Paper - VIII : PLANT BIOTECHNOLOGY 

Time : 03 Hours                                                                            Maximum Marks : 100

Section - A (5 × 8 = 40) 

Answer any Five of the following 
1) Micropropagation. 
2) Culture medium. 
3) Synthetic seeds. 
4) Somatic hybridization. 
5) Gene manipulation. 
6) PCR. 
7) Biolistic method of gene transfer. 
8) PVC vector. Section - B (4 × 15 = 60) 
Answer All of the following 9) a) Describe the methods of androgenic haploid production and their uses. 
OR 
b) Explain the method of in vitro selection of mutants. 
10) a) What is biotransformation? Explain with the help of suitable examples. 
OR 
b) What is somatic hybridization? Describe the various techniques used for protoplast 
fusion. 11) a) Explain the technique of Western blotting and its uses. 
OR 
b) Distinguish between genomic and cDNA libraries. Explain the method of construction 
of cDNA library. 
12) a) Write an essay on the application of gene transformation techniques in crop 
improvement. 
OR 
b) Distinguish between RAPD and RFLP. Describe the use of RAPDs in gene mapping. 
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