B.Sc-B.Sc MLT 1st Year Basic Principles of Biochemistry(Baba Farid University of Health Sciences, Faridkot, Punjab-2013)
B.Sc.(M.L.T.) [1st Year]
BF/2013/06
Basic Principles of Biochemistry
(Old Scheme - Prior to admission 2009)
M.M. : 100 Time : 3 Hours
Note: Attempt all questions.
USE SEPARATE ANSWER SHEET FOR EACH PART.
PART – I
1. What are Buffers ? Describe in detail the preparation of 200 ml of Phosphate
Buffer (M/15, pH 7.45) and determination of pH by pH Meter. [20]
2. Write Short Notes on :
(a) Benediet's Test for reducing sugars. [5]
(b) Define Serum, Plasma and Whole Blood. [6]
(c) Describe a pH Meter, with the help of a diagram. [4]
3. (a) What are Bile Pigments ? How are they detected in Urine and what is the
clinical significance of detection of Bile Pigments in Urine. [10]
(b) How will you prepare 100 ml normal Saline (0.9N, NaOH) in Laboratory. [5]
PART –II
4. Write the method of collection of Urine for 24 Hours Urinary Protein estimation.
Write the clinical significance of Proteinuria. [15]
5. Write Short Notes on : [4x5=20]
(a) Benedict's Reagent.
(b) Porphobilinogen.
(c) Buffers.
(d) Heat Coagulation Test.
6. (a) Enumerate the different qualitative tests for detection of reducing substances
in Urine. Compare them in terms of sensitivity and specificity. [10]
(b) Write a short note on normal range and reference range. [5]
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| Earning: Approval pending. |
