B.Sc-B.Sc MLT 1st Year Basic Principles of Biochemistry(Baba Farid University of Health Sciences, Faridkot, Punjab-2013)
B.Sc.(M.L.T.) [1st Year]
BF/2013/11
Basic Principles of Biochemistry
(Old Scheme - Prior to admission 2009)
M.M. : 100 Time : 3 Hours
Note: Attempt all questions.
USE SEPARATE ANSWER SHEET FOR EACH PART.
PART – I
1. Describe the principle of Benedict’s test for reducing substances in urine. How will you prepare Benedict’s qualitative reagent in the laboratory. [20]
2. Write Short Notes on : [5x4=20]
(a) pH Meter .
(b) Osmosis.
(c) Dialysis.
(d) Analytical Balance.
3. What is Ketonuria ? What are the conditions in which Ketonuria occurs and how is Ketonuria detected.
How are they detected in Urine and what is the
clinical significance of detection of Bile Pigments in Urine. [10]
(b) How will you prepare 100 ml normal Saline (0.9N, NaOH) in Laboratory. [5]
PART –II
4. Write the method of collection of Urine for 24 Hours Urinary Protein estimation.
Write the clinical significance of Proteinuria. [15]
5. Write Short Notes on : [4x5=20]
(a) Benedict's Reagent.
(b) Porphobilinogen.
(c) Buffers.
(d) Heat Coagulation Test.
6. (a) Enumerate the different qualitative tests for detection of reducing substances
in Urine. Compare them in terms of sensitivity and specificity. [10]
(b) Write a short note on normal range and reference range.
| Earning: Approval pending. |
