M.Sc-M.Sc Bio Chemistry 2nd Sem BCH - 273 : Membrane Biochemistry and Genetics(University of Pune, Pune-2013)
M.Sc. (Semester - II)
BIOCHEMISTRY
SEAT No. :
[Total No. of Pages : 2
BCH - 273 : Membrane Biochemistry and Genetics (2010)
Membrane Biochemistry and Nucleic acids (2008)
Time : 3 Hours] [Max. Marks : 80
Instructions to the candidates :
1) 2) 3)
All questions are compulsory.
Figures to the right indicate full marks.
Answers to the two sections should be written in separate answer books.
SECTION - I
(Membrane Biochemistry)
Q1) Answer any three of the following : [15]
Structural features and role of gramicidin as a transport antibiotic.
Lipids as a major constituent of biological membranes.
Structure and significance of bacterial cell wall.
Types of transport process. Compare these with respect to energetics
involved.
Q2) Answer any three of the following : [15]
a) Sodium channel and its significance.
b) Calcium pump and its physiological significance.
c) ABC transporters and their role.
d) Cotransport of chloride and bicarbonate in humans.
P.T.O.
Q3) Write short notes on any two : [10]
Phosphotransferase system.
Gap junctions and its significance. Nuclear pore complex.
SECTION - II
Nucleic Acids (Old) Genetics (New)
Q4) Answer any three of the following : [3 × 5 = 15]
a) Discuss the experimental evidence that prove DNA as genetic material.
b) Differentiate between transformation and transduction.
c) Define vectors. Give their significance in gene cloning.
d) Enlist the salient features of Watson and Crick model of DNA.
Q5) Answer any three of the following : [3 × 5 = 15]
a) Elaborate on Messelson and stahl experiment and its interpretation.
b) Comment on denaturation and renaturation of DNA.
c) Explain the significance of structural and regulatory genes of Lac. Operon.
d) Enlist the features of genetic code.
Q6) Write note on any two of the following : [2 × 5 = 10]
a) Complementation tests.
b) Mutation and genetic disorders.
c) Mendelian laws of inheritance.
| Earning: Approval pending. |
