M.Sc-M.Sc BioTechnology 1st Sem BT-11 : Advanced Biological Chemistry(University of Pune, Pune-2013)
M.Sc. (Semester - I)
SEAT No. :
[Total No. of Pages : 2
BIOTECHNOLOGY
BT-11 : Advanced Biological Chemistry
(2008 Pattern)
Time : 3 Hours] [Max. Marks :80
Instructions to the candidates :
1) Attempt not more than 5 questions of which at least 2 questions must be from
each section.
2) Answers to the two sections should be written in separate books. 3) Neat diagrams must be drawn wherever necessary. 4) Figures to the right indicate full marks. 5) All questions carry equal marks.
SECTION - I
Q1) a) What are biological buffers? Explain bicarbonate system for maintaining
acid - base balance. [8] b) Give principle and application of ISO - electric focussing. [8]
Q2) a) Differentiate between analytical and preparative centrifugation with suitable
example. [8]
b) Explain the term metabolic engineering with representative example. [8]
Q3) a) Discuss the physical basis of peak broadening in chromatography with
example. [8]
b) Give the types of secondary metabolites of plant origin. State the use of
any one type in agriculture. [8]
Q4) Write short notes on : [16]
a) Electroendoosmosis.
b) Metabolic flux analysis.
c) Chemical shift.
d) Redox potential.
P.T.O.
SECTION - II
Q5) Describe the non covalent forces that control the protein structure with
illustrative example. [16]
Q6) a) Discuss the importance of multienzyme complexes in natural product
biosynthesis. [8]
b) Describe Jacques Monads (MWC) model for allosteric transitions of
protein. [8]
Q7) a) Enlist the types of terpenoids and give the steps involved in biosynthesis
of terpenoids. [8]
b) Explain the role of proteins in membrane organisation. [8]
Q8) Write short notes on : [16]
Use of herbals in medicine. Protein engineering.
Chow - Fasman method.
Pharmacological activities of phenolics.
| Earning: Approval pending. |
