M.Sc-M.Sc BioTechnology 3rd Sem BT - 31 : Tissue Culture (Plant and Animal)(University of Pune, Pune-2013)
M.Sc. (Semester - III) BIOTECHNOLOGY
SEAT No. :
[Total No. of Pages : 1
BT - 31 : Tissue Culture (Plant and Animal)
(2005 Pattern)
Time : 3 Hours] [Max. Marks : 80
Instructions to the candidates:
1) Attempt a total of Five questions selecting atleast two questions from each section.
2) Answers to the sections must be written on separate answer books.
3) Neat diagrams must be drawn wherever necessary.
4) Figures to the right indicate full marks.
SECTION - I
Q1) What is micropropagation? Mention its stages, advantages and limitations.[16]
Q2) Enlist the incubation systems used for plant tissue and cell culture. Explain
any one system. Add a note on its advantages. [16]
Q3) Explain the procedure for obtaining plant protoplasts. Enlist the applications
of isolated plant protoplasts and explain any one.
Q4) Write explanatory notes on any two of the following.
a) Redifferentiation in vitro.
b) Hormonal regulation of in vitro morphogenesis.
c) GM crops
SECTION - II
Q5) Explain advantages and limitations of serum free media in animal cell &
tissue culture. [16]
Q6) How are three dimensional cultures established and maintained? What are
the advantages and applications of three dimensional cultures? [16]
Q7) Explain the basis and procedure of cryopreservation. What are its applications
in animal cell and tissue culture?
Q8) Write explanatory notes on any two of the following.
a) Stem cell culture.
b) Maintenance of cell lines. c) Cell disaggregation.
| Earning: Approval pending. |
