M.Sc-M.Sc BioTechnology 3rd Sem BT-32 : Fundamentals of Genetic Engineering(University of Pune, Pune-2013)
M.Sc. (Semester - III) BIOTECHNOLOGY
BT-32 : Fundamentals of Genetic Engineering
(2005 Pattern)
Time : 1½ Hours] [Max. Marks :40
Instructions to the candidates :
1) Attempt a total of four questions selecting atleast Two Questions from each
section.
2) Answers to the sections must be written on separate answer books. 3) Neat diagrams must be drawn wherever necessary. 4) Figures to the right indicate full marks.
SECTION - I
Q1) What is a cDNA library? Explain the method for construction of cDNA library.
[10]
Q2) What are expression vectors? Mention such vectors in bacteria and describe
salient features of any one such vector.
Q3) Write notes on
a) Site directed mutagenesis. b) Southern blotting.
SECTION - II
Q4) Explain the method of screeing and selection of transformant. Cite an
appropriate example. [10]
Q5) Enlist the methods of transformation in vitro. Explain any one method to
obtain genetically transformed plant cells.
Q6) Write notes on.
a) YAC
b) Chimeric constructs.
ttt
| Earning: Approval pending. |
