M.Sc-M.Sc BioTechnology 2nd Sem BT-21 : Molecular Biology(University of Pune, Pune-2013)
M.Sc. (Semester - II)
SEAT No. :
[Total No. of Pages : 2
BIOTECHNOLOGY
BT-21 : Molecular Biology
(2005 Pattern)
Time : 3 Hours] [Max. Marks :80
Instructions to the candidates :
1) Question Nos. 1 is compulsory. Out of the remaining attempt 4 questions.
2) Neat diagrams must be drawn wherever necessary.
3) Figures to the right indicate full marks.
Q1) a) Give the importance of DNA repair mechanisms. [5]
b) Explain the genetic code used for protein synthesis. [5]
c) Differentiate between the protooncogenes and oncogenes. [5]
d) Explain chromatin remodelling in relation to gene expression. [5]
Q2) a) Give the mechanism of action of DNA Polymerase during replication.
Add a note on the types of polymerases. [8]
b) Discuss the mitochondrial genome with reference to nuclear genome.[7]
Q3) a) How is the mechanism of translation in prokaryotes different from
eukaryote [8]
b) Explain the enzymes involved in DNA modification. Give their importance.
[7]
Q4) Write short notes on : [15]
a) Sequence complexicity in eukaryote
b) Chemical agents causing DNA damage.
c) DNA melting and buoyant density.
P.T.O.
Q5) a) Describe the techniques used to understand DNA - protein interaction.[8]
b) Describe the homeotic gene complexes that determine pattern formation
in Drosophillo. [7]
Q6) a) Define recombination. Give a comparative between homologous and
non - homologous recombination. [8]
b) Explain the structure and mechanism of action of RNA polymerases.[7]
| Earning: Approval pending. |
