M.Sc-M.Sc BioTechnology 3rd Sem BT-33 : Biological Chemistry - II(University of Pune, Pune-2013)
M.Sc. (Semester - III)
SEAT No. :
[Total No. of Pages : 1
BIOTECHNOLOGY
BT-33 : Biological Chemistry - II
(2005 Pattern)
Time : 1½ Hours] [Max. Marks :40
Instructions to the candidates :
1) Attempt a total of four questions selecting atleast Two Questions from each
Section.
2) Answers to the Sections must be written on separate answer books. 3) Neat diagrams must be drawn wherever necessary. 4) Figures to the right indicate full marks.
SECTION - I
Q1) What is electrophoresis? Mention its types and explain any one. [10]
Q2) Explain the use of ion exchange chromatography for purification of proteins.
[10]
Q3) Write notes on : [10]
a) Ramchandran plot.
b) Protein sequencing
SECTION - II
Q4) Explain the salient features of tertiary structure of proteins. [10]
Q5) How is differential centrifugation employed for cell fractionation? Add a note
on isolation of any one cell fraction.
Q6) Write notes on :
a) MALDI. TOF b) Micro arrays.
| Earning: Approval pending. |
