M.Sc-M.Sc BioTechnology 3rd Sem BT - 32 : Fermentation Technology(University of Pune, Pune-2013)

                                      M.Sc. (Semester - III)

SEAT No. :

[Total No. of Pages : 2

BIOTECHNOLOGY

BT - 32 : Fermentation Technology

(2008 Pattern)

Time : 3 Hours]                                                                                            [Max. Marks :80

Instructions to the candidates :

1) Attempt a total of five questions selecting atleast two questions from each

section.

2) Answers to the two sections must be written in separate books. 3) Neat diagrams must be drawn wherever necessary. 4) Figures to the right indicate full marks.

SECTION - I

Q1) a)      Discuss mass transfer by molecular diffusion in a fermenter.        [8]

b)       Discuss the kinetics of product formation by microbial culture in terms

of growth linked products in batch and fed batch cultures.            [8]

 Q2) Explain why biotransformation is widely seen as Green alternative over

conventional chemical routes. Outline the difference between cell and enzyme based biotransformation.

What is measure of volumetric oxygen transfer rate in a fermenter? How

KLa is derived in a fermenter.

[16]

Q3)Describe significance of membrane filtration over centrifugation.

Discuss role of bio - control agents in integrated pest management. What

are challenges in terms of acceptance of the same?

[16]

Q4) Explain isolation of products of biotransformation with suitable example.

What is Reynold’s number? How it can be useful for to characterize

fluid flow?

[16]

P.T.O.

Q5)  Which separation method is suitable for purification of monoclonal

antibodies? What are the advantages of affinity chromatography over

size exclusion chromatography?

Discuss different physical and chemical sensors in a fermenter?

[16]

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