M.Sc-M.Sc BioTechnology 2nd Sem OtherBT-23 : Plant Biotechnology(University of Pune, Pune-2013)

                                  M.Sc. (Semester - II) BIOTECHNOLOGY

SEAT No. :

[Total No. of Pages : 2

BT-23 : Plant Biotechnology

(2008 Pattern)

Time : 3 Hours]                                                                                                     [Max. Marks : 80

Instructions to the candidates:

1) Attempt a total of five questions selecting atleast two questions from each section.

2) Answers to the two sections must be written on separate answer books.

3) Neat diagrams must be drawn wherever necessary.

4) Figures to the right indicate full marks.

SECTION - I

Q1) With a suitable example explain the methods used for increased production

of secondary metabolites in vitro using plant cell cultures. Give the significance

of in vitro method over the in viva.                                                               [16]

Q2) a) Comment on the methods used for seed improvement, testing and

certification.                                                                                                         [8]

b) Explain how clonal variations has helped the crop improvement

programme using plant tissue culture technique.                                [8]

Q3) a) Explain qualitative and qvantitative improvement of economically

important fungi using modern techniques.                                            [8]

b) Give the traditional uses of algae. Discuss use of biotechnology for mass

propagation of commercially important algal species.

Q4) Write short notes on :

a) Plant growth regulators. b) Somatic embryogenesis.

c) Biodiesel

d) Haploid production.

SECTION - II

Q5) a) Explain the biolistic - mediated DNA transfection of monocot plants for

pest resistant transgenic plant.                                                                  [8]

b) Give the importance of use of biofertilizers and vermiculture in modern

agriculture practices.                                                                                     [8]

P.T.O.

Q6) What is plant transformation. With suitable example, explain the direct and indirect

methods of DNA transfer to plants to produce the transgenic plants.              [16]

Q7) a) Define the term molecular farming? Explain how plants can be used for

production of pharmaceutical and cosmaceutical products.           [8]

b) Describe the process of protoplast isolation, fusion and regeneration of

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