M.Sc-M.Sc Bio Chemistry 3rd Sem BCH - 370 : Molecular Biology(University of Pune, Pune-2013)
M.Sc. (Semester - III)
BIOCHEMISTRY
SEAT No. :
[Total No. of Pages : 2
BCH - 370 : Molecular Biology
(2010 Pattern and 2008 Pattern)
Time : 3 Hours] [Max. Marks : 80
Instructions to the candidates:
1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Draw neat labelled diagrams wherever necessary.
Q1) Answer any four of the following: [20]
What is the mechanism of action of topoisomerase II enzyme on DNA?
What is Okazaki fragment? How is it synthesized?
Explain the role of Ruv system in resolving Holliday junction.
Give an account on RNA polymerase structure and role from E.Coli.
Describe structure / features of t-RNA molecule and its role.
Q2) Answer any two of the following : [20]
Describe in detail spliceosome mediated splicing event in eukaryote system.
Discuss the structure and arrangement of nucleosome in chromatin.
What is signal hypothesis? Give the detailed mechanism involved in protein
targeting.
P.T.O.
Q3) Give a short account on the following (any four) : [20]
Telomeres and its significance.
SOS response.
Involvement of plasmids in antibiotic resistance.
Antibiotics inhibiting protein biosynthesis in prokaryotes.
Cot curve and its significance.
Q4) Write short notes on any four : [20]
Compare and contrast prokaryotic and eukaryotic ribosomes.
Structural aspects of H1N1 virus.
Targeting of proteins to nucleus.
Types of mutations and its consequences.
Initiation of transcription in prokaryotes.
TTT
| Earning: Approval pending. |
