M.Sc-M.Sc Bio Chemistry 4th Sem BIOCHEMISTRY(University of Pune, Pune-2013)

                                  [4326] - 401

                                                 M.Sc. (Semester - IV)

                                                                BIOCHEMISTRY

SEAT No. :

[Total No. of Pages : 3

BCH - 470 : Biochemical Endocrinology and Tissue culture (2008 Pattern)

Biochemical Endocrinology and Plant Biochemistry (2010 Pattern)

Time : 3 Hours]                                                          [Max. Marks : 80

Instructions to the candidates:

1) All questions are compulsory.

2) Draw neat diagram wherever necessary.

3) Figures to the right hand side indicate full marks.

4) Answers to the two sections should be written on separate answer books.

SECTION - I

Biochemical Endocrinology

Q1) Answer any three of the following:                                                                  [15]

a) Briefly describe the production of T4 & T3 including the following terms:

i) iodide / iodine.

ii) thyroglobulin (TGB) iii) colloid.

iv) tyrosine residues.

v) thyroxine - binding globulin (TBG).

b) Write note on the biological role of posterior pituitary hormones.

c) What are the hormones secreted by the adrenal cortex? What are their

respective functions?

d) Describe the mechanism to control the secretion of insulin.

Q2) Attempt any three of the following:                                                                 [15]

a) What are prostaglanding? Write note on their functions.

b) Elaborate on the biosynthesis of male and female sex hormones.

c) What are the clinical manifestations of growth hormone deficiency? d) Write a note on gastrointestinal hormones.

P.T.O.

Q3) Answer any two of the following :                                                                   [10]

Write a note on zinc fingurs.

How G-proteins regulate the activity of adenylate cyclase?

Discuss how the negative feedback mechanism helps to maintain proper

balances of hormones in the blood.

SECTION - II

Tissue Culture (Old)

Q4) Answer any three of the following:                                                                  [15]

Explain the role of growth regulators.

Explain the process of micropropogation. Give the various ways of sterilization.

Describe the process of anther culture. Explain the term cybrides.

Q5) Answer any three of the following:                                                                  [15]

Give the advantages and disadvantages of natural media.

What is primary culture? How it is obtained?

Give the importance and limitation of organ culture. Discuss the characteristics of transformed cells.

Describe the various culture methods involved in animal tissue culture.

Q6) Write short notes on any two of the following :                                          [10]

a)       Cloning.

b)       Cell preservation.

c)       Hairy root culture.

d)       Secondary metabolites.

[4326]-401                                                    2

SECTION - II

Plant Biochemistry (New)

Q4) Attempt any three of the following:                                                                 [15]

Localization of photosystems in thylakoid membrane. Role of phosphorous in plant growth.

Functions of Gibberellic acid. Calvin cycle.

Role of ethylene oxide in fruit ripening.

Q5) Explain the following (any three) :                                                                   [15]

Symbiotic nitrogen fixation. Micropropagation.

Somatic hybridization. Anthocyanins.

Role of magnesium in plant growth.

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