B.Sc-B.Sc Microbiology 1st Sem Basic Techniques in Microbiology(University of Pune, Pune-2013)
F.Y. B.Sc.
MICROBIOLOGY
SEAT No. :
[Total No. of Pages : 2
Basic Techniques in Microbiology
(2008 Pattern) (Paper - II)
Time : 3 Hours] [Max. Marks : 80
Instructions to the candidates:
1) All questions are compulsory.
2) Draw neat labelled diagrams wherever necessary.
3) Figures to the right indicate full marks.
Q1) Attempt the following
a) What is astigmatism?
b) Match the following and rewrite
i) NH4Cl 1) ‘C’ source
ii) Glucose 2) maintains osmotic pressure
iii) NaCl 3) maintains pH
iv) Buffers 4) ‘N’ source
c) ____ is an acid fast bacterium and its cell wall contains _____.
d) Mention the pressure, time and temperature regime for sterilization in
autoclave.
e) Name two methods of maintenance of fungal culture. f) Enlist growth phases in liquid batch culture.
g) In an electron microscope, source of illumination is ______ and lenses
are _____.
h) What are photo autotrophs. Give one example.
Q2) Write short note on (Any four) :
a) Possible hazards in Microbiology Laboratory.
b) Numerical aperture.
c) Accentuators.
d) Cultivation of photosynthetic organism.
e) Culture collection centre.
f) Diauxic Growth.
Q3) Attempt any four of the following :
a) Justify - Light rays of short wavelength give better images.
b) What is special staining? Explain method of capsule staining.
c) Comment on use of filtration as a sterilization agent.
d) Explain - Enrichment and enriched media.
e) Differentiate in tabular form - chemostat and turbidostat.
f) What is synchronous culture? Explain any one method for obtaining it.
Q4) Attempt any two of the following : [16]
a) What is differential staining? Describe the principle, procedure and
significance of Gram staining.
b) What are differential and selective media? Explain their significance with
suitable example.
c) Enlist methods for enumeration of bacteria. Explain any two methods in
detail.
d) What is pure culture? Describe any one method of obtaining pure culture.
Q5) Attempt any one of the following : [16]
a) With proper ray diagram explain principle, working and applications of
phase contrast microscope.
b) What is an ideal disinfectant? Explain the mechanism of action of any
four chemical agents.
| Earning: Approval pending. |
