B.E-B.E Biotechnology 1st Sem GENETIC ENGINEERING(University of Pune, Pune-2013)

           UNIVERSITY OF PUNE

[4363-271]

T.E.(Biotechnology) Examination,2013

GENETIC ENGINEERING

(2008 pattern)

Time-Three hours                                                                Maximum Marks-100

[Total No. of Question=12]                                     [Total no. of printed pages= 3]

Instructions:

(i)Answer Q. No. 1 or Q. No. 2, Answer Q. No. 3 or Q. No. 4, Answer Q. No.

5or Q. No. 6, Answer Q. No. 7 or Q. No. 8, Answer Q. No. 9 or Q. No. 10, Answer Q. No. 11 or Q. No. 12.

(ii)Answers to the two sections should be written in separate answer books. (iii)Draw neat diagrams wherever necessary.

(iv)Figures to the right indicate full marks.

SECTION-I

Q.1. Describe and discuss in detail the procedure for Southern Blotting with a neat

labelled diagram also give its application.                                                            (18)

OR

Q.2      Describe RTPCR and give its application. Enlist and describe the usage of the

various enzymes involved in RTPCR and PCR.                                                (18)

Q.3      What are Cloning vectors, explain their significance in genetic engineering?

Describe a model plasmid cloning vector?                                                           (16)

OR

Q.4      What are expression vectors? Give their significance , and describe a typical

expression vector.                                                                                                         (16)

Q.5      Explain the concept of genomic libraries, how are they constructed? What are

cDNA libraries?                                                                                                                 (16)

OR

Q.6      Write in detail the steps involved in cloning a protein product till mass production.

(16)

SECTION-II

Q.7      Answer the following (9 marks each)                                                                    (18)

(i)Explain in detail screening of recombinants using the blue white selection method.

(ii)Describe PCR cloning.

OR

Q.8      Explain the dideoxy sequencing method in detail, with a neat diagram.   (18)

Q.9      What are the different ways by which genomic material is transferred in bacteria?

Explain with respect to Conjugation and transformation.                             (16)

OR

Q.10 How are transgenics organisms constructed? What are their significance and

applications in biotechnology?                                                                                 (16)

Q.11 Answer the following (8 marks Each)

(i)Explain the application of recombinant DNA Technology, in agriculture with a detailed analysis of golden rice.

(ii)What is Human Genome Project?How was it impacted biology and

biotechnology?

OR

Q.12 Write a short note on (4 marks each)

(i) RFLP (ii) AFLP

(iii) DNA diagnostics

(iv) Hep B

(16)

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