B.E-B.E Biotechnology 1st Sem Fermentation Technology - II(University of Pune, Pune-2013)

[4363]-277

T. E. Biotechnology, Examination - 2013

Fermentation Technology - II

(2008 Course)

[Total No. of Questions: 12]                                [Total No. of Printed Pages: 2]

[Time: 3 Hours]                                                                          [Max. Marks: 100]

Instructions:

(1) Answer any three questions from each section-I and any

three from section-II.

(2) Figures to the right indicate full marks.

(3) Use of programmable calculator is allowed.

(4) Assume suitable data, if necessary.

SECTION-I

Q. 1. Explain the factors effecting diffusion in Bio processing.

OR

Q. 2. Write short notes on the following.

i. Impeller Reynolds number

ii. Power number

Q. 3. Write short notes on Bubble driven Bioreactors.

OR

Q. 4. Briefly explain the Construction and working of Continuous

Countercurrent Decanter.

Q. 5. Define Adsorption. What is its application in Biotech Industries?

Explain how the nature of adsorbents affects the efficiency of a process?

OR

Q. 6. Prove that two stage crosscurrent treatment of liquid solution by

Contact filtration when the adsorption isotherm is linear, the least

Total adsorbent results if the amounts used in each stage are equal.  (18)

SECTION-II

Q. 7. List out the equipments used for leaching of fine solids. Explain the

Constructions and working of Ball man Extractor.

OR

Q. 8. Soybean oil is to be extracted from granulated soybean seeds in a

Countercurrent extraction unit using ethyl ether as a solvent. The

Mass flow rate of halibut seeds to the unit is 350kg/hr. The fresh seeds

contain 20% oil and are to be extracted to a composition of 1% of oil. The

Solvent containing 2% oil to be used as used as a solvent is fed to the unit at The rate 250kg/hr. Determine the number of theoretical stages required. And % recovery of oil.

Concentration           0            0.1 0.2 0.3 0.4 0.5 0.6

Solution retained 0.28 0.34 0.4 0.47 0.55 0.66 0.8

Q. 9. What is membrane fouling? Explain the factors which decrease the

Flux through a membrane. How can it be prevented?

OR

Q. 10. Explain the parameters which are used to assess Pervaporation

Process?

Q. 11. Define liquid Extraction. What are the fields of its usefulness?

OR

Q. 12. What are the factors affecting the Choice of a solvent in any extraction (18)

Operation? Explain how each factor actually affects the efficiency of

the operation?

2

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