B.Sc-B.Sc MLT 4th Year HISTOTECHNOLOGY AND CYTOGENETICS (Kerala University of Health Sciences-2014)

QP CODE: 403012                                              Reg No:………………….
                       Final Year B.Sc MLT Degree Examinations, September 2014
                                       HISTOTECHNOLOGY AND CYTOGENETICS

Time: 3 Hours                                                                   Total Marks: 100

• Answer all Questions.
• Draw Diagrams wherever necessary.

 

Essay (2x10=20)

1. Explain the principle and methodology in processing of tissues for histopathology. Describe the various parts and care of an automatic tissue processor (2+3+5=10)

2. Mention the various methods of chromosome analysis. Describe the cytogenetic abnormalities in myeloid leukemia. Discuss briefly the use of FISH in chromosome analysis (3+3+4=10)

 

Short notes (10x5=50)

3. Methods of decalcification

4. Frozen sections

5. Rapid tissue processing

 

6. Banding techniques

7. Types of microtomes and its uses

8. Fungal stains

9. Tissue processing for electron microscopy

10. Fixatives in histopathology

11. Role of histopathology laboratory in disease diagnosis

12.Types of haematoxylin stains and its uses

 

Answer briefly (10x3=30)

13. Vacuum embedding

14. Mucin stains

15. Silver stains

16. Demonstration of lipid in tissues

17. Knife sharpening

18. Mordant

19. Barr body

20. Resin embedding

21. Types of disposable microtome blades

22. Identification of pigments in tissue

 

******************

Earning:  ₹ 7.25/-