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B.E-B.E Biotechnology 1st Sem Microbiology (University of Pune, Pune-2013)

Sunday, 28 September 2014 12:52Nitha

                                 UNIVERSITY OF PUNE
                                       [4362]-233
                      S. E. (Biotechnology)  Examination - 2013
                                       Microbiology
                                        (2008 Pattern)

 


Total No. of Questions : 12                [Total No. of Printed Pages :2]

[Time : 3 Hours]                                                                [Max. Marks : 100]

Instructions :
(1) Answer Q1 or Q2, Q3 OR Q4, Q5 OR Q6, Q7 OR Q8, Q9 OR Q10, Q11 OR Q12.
(2) Answers to the two sections should be written in separate  answer-books.
(3) Neat diagrams must be drawn wherever necessary.



SECTION-1


Q1. Enumerate the growth requirement of bacteria. Describe in detail the method  of anaerobic culture. [18]
OR
Q2. What are the similarities and differences between prokaryotic and eukaryotic cells? [18]

 

Q3. Answer the following: (8 marks each) [16]

1) What are the three domains of life?

2) Describe the construction and working of a Hot Air Oven.

OR

Q4. Answer the following: (8 marks each) [16]

1) Describe the important contribution of Antony Van leeuwenhoek.

2) Explain the structure of cell membrane.

 

Q5. Define each of these groups as what kind of carbon and energy source they utilize: Give examples. [16]

1) Phototrophs

2) Lithotrophs

3) Organotrophs


4) Hetetotrophs

OR

Q6. Answer the following: (8 marks each) [16]

1) Differentiate between macronutrients and micronutrients. How are they supplied to media?

2) Explain the typical bacterial growth curve.

 

Section-II

Q7. What is a bacteriophage? Draw a diagram of the T4 bacteriopahge an explain its life cycle. [18]

OR

Q8. Explain the Egg inoculation technique in virology. [18]

 

Q9. Answer the following: (8 marks each) [16]

1) What is food spoilage? Explain its causes.

2) Explain the symbiotic association found in soil microorganisms.

OR

Q10. Answer the following: (8 marks each) [16]

1) Write the causative agent of tuberculosis and explain the morphology of the organism.

2) List the characteristics common to all viruses.

 

Q11. Answer the following: (4 marks each) [16]

1) Drug resistance.

2) Lysogeny

3) Water borne illness

4) Anaerobic sewage treatment.

OR

Q12. Write in brief: (8 marks each) [16]

1) Foods for microorganisms

2) Techniques used for isolation of microorganisms from air.



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