B.Tech-B.Tech Biotechnology 1st Sem Biochemistry-I (University of Pune, Pune-2013)

                                               PUNE UNIVERSITY
                                                    [4362]-234
                                    S. E. (Biotechnology) Examination
                                                    M a y 2013
                                                    Biochemistry-I
                                                       (2008 Course)

 

[Total No. of Questions: 12]                               [Total No. of Printed Pages: 3]

 

[Time: 3 Hours]                                                                        [Max. Marks: 100]

 

Instructions:

(1) Answer three questions from each section-I and three  questions from section-II.
(2) Answers to the two sections should be written in separate answer-books.
(3) Neat diagrams must be drawn wherever necessary.
(4) Figures to the right indicate full marks.

SECTION-I

Q. 1. Answer the following (18)

A) Write short notes on carbonate bicarbonate buffering system in blood.

B) Calculate the pKa of lactic acid, given that when the concentration  Of lactic acid is 0.010 M and the concentration of lactate is 0.087 M, the pH is 4.80.

OR

Q. 2. Answer the following (18) 

 

A) Draw a diagram showing the hierarchy of biomolecules and Organelles  in the living cell.

B) Write a short note on storage polysaccharide

 

Q. 3. Answer the following

A) Draw a neat labeled diagram for a) Cori cycle b) TCA cycle (16)

OR 

Q. 4. Describe the synthesis of starch. Explain the role nucleoside (16) diphosphate sugars in the same.

 

Q. 5. Write in short about (16)

A) Acid base properties of amino acid

B) Affinity and size exclusion chromatography

OR

Q. 6. Answer the following (16)

A) Draw the structures of peptide bone and explain its typical Characteristics 

B) Draw a schematic for Urea cycle

 

SECTION-II

Q. 7. Answer the following (18)

A) Biosynthesis of sphingomyelin

B) Elongation of saturated fatty acids in mitochondria

OR

Q. 8. Answer the following (18)

A) What are ketone bodies? Explain the role of acetyl-CoA in the Formation of ketone bodies. 

B) Write in detail about classification of lipids with suitable example

 

Q. 9. Write short notes on (16)

A) Nucleoside and nucleotide

B) Purines and pyrimidines 3

OR

Q. 10. Describe in detail about (16)

A) Hydrolysis of nucleic acid by enzymes

B) Nucleic acid and protein supramolecular complex

 

Q. 11. Write down the sources and functions of vitB1,B2,B6,B12, vit C (16)

OR

Q. 12. Answer the following (16)

A) Describe in detail about the function and deficiencies of fat soluble Vitamins

B) What are fibers? Describe the different types of dietary fibers and  Give its application. 1

T

Earning:  ₹ 8.10/-