B.Tech-B.Tech Biomedical Engineering 2nd Sem BBTZ01 LIFE SCIENCE(Dr MGR Educational and Research Institute, Chennai-2013)
B.TECH DEGREE EXAMINATIONS,
MAY/ JUNE- 2013
Subject Code- BBTZ01 LIFE SCIENCE
Time: three hours Maximum Marks: 100
Part A (10 X 2= 20 Marks)
Answer All Questions
1. Define cell lines.
2. Write the role of rough endoplasmic reticulum.
3. Name any two restriction enzymes and write its specific restriction site.
4. What is the role of DNA gyrase?
5. Write the applications of ELISA.
6. Write the importance of ammonium persuplhate in SDS PAGE.
7. Define fermentation.
8. What are monoclonal antibodies?
9. Define numerical aperture.
10. Write the use of dark field microscope.
Part B (5 X 16= 80 Marks)
Answer All Questions
11. Describe the electron transport chain for ATP generation.
(or)
12. Write the applications for animal and plant cell culture.
13. Elaborate the transcription process of the prokaryotic cell.
(or)
14. Explain the genes and regulation of lac operon.
15. Describe the principle, applications and procedure for SDS PAGE.
(or)
16. Explain the principle, procedure and applications for agarose gel electrophoresis.
17. Elaborate the downstream operations in fermentation process.
(or)
18. Explain the production and industrial applications of protease enzyme.
19. Describe the working principle and applications of phase contrast and fluoroscent microscope.
(or)
20. Elaborate the working principle and applications of electron microscope.
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