North Maharashtra University 2008 B.Sc Biotechnology S.Y.- - CELL BIOLOGY AND BASIC METABOLISM - exam paper

-----------------------------------------------------------------
NORTH MAHARASHTRA UNIVERSITY, JALGAON

ques. Paper :- S.Y.BSc. - BIOTECHNOLOGY

BT 211 & 212 2008-09


CELL BIOLOGY AND BASIC METABOLISM
SEMESTER- FIRST
-----------------------------------------------------------------

1) ……………. is a DNA sequence that regulates transcription of
structural genes.
a) Regulatory site b) Promoter site
c) Operator site d) Repressor site.
2) Operon consists of ……….. cluster.
a) Protein coding genes b) Structural genes
c) Non coding genes d) none.
3) Regulatory protein is also known as ………... of operon.
a) Inhibitor b) Activator
c) Repressor d) Controller.
4) ……………is an inducer of the lac operon.
a) Lactose b) cAMP
c) ATP d) Allolactose.
5) The lac operon is a good example of ……………...
a) Stringent control b) Negative control
c) Positive control d) None.
6) Structural genes of operon encodes for…………
a) Repressor molecule b) Inducer proteins
c) Regulatory enzymes d) Activator molecule.
7) ………. Is binding site of RNA polymerase on sequence of DNA.
a) Operator b) Binding site
c) Regulator d) Promoter.
8) ……….. kind of mRNA transcribed by RNA pol. In Lac operon.
a) Polycistronic b) Monocitronic
c) Dicistronic d) Multivalent.
9) The enzyme………..hydrolyzes lactose to glucose & galactose.
a) Galactosidase b) Galactosidase.
c) Lactose permease d) lac convertase
10) Concept of Operon wag provided by ……….&………..
a) Beadle & Tatum b) Broom & Gillbert
c) Jacob & Monad d) Richadr & Philip.


Q.2 ques. for two Marks
1) provide the role of 3 enzyme of Lac-operon.
2) Comment on importance of cAMP in Lac operon.
3) elaborate structural genes? discuss their role.
4) describe Promoter & Operator.
5) What is operon? discuss its concept briefly.
6) Compare positive & negative regulation.
7) provide the role of allolactose in lac operon.
8) provide the importance of regulatory genes.
9) Enlist enzymes of lac operon & comment on their role.
10) describe polycistronic mRNA.


Q.3 ques. for three Marks.
1) Comment on concept of operon.
2) What is structural gene? discuss in details.
3) define Regulatory gene?
4) Comment on Lac repressor protein.
5) discuss promoter & operator of operon.
6) Compare Polycistronic & Monocistronic mRNA.
7) provide significance of an Inducer in Lac operon.
8) describe Operon? Enlist Enzymes of Lac operon with their role.

Q.4 ques. for four Marks.
1) Distinguish ranging from Positive & Negative Regulation.
2) Comment on Regulation of operon.
3) What is Promoter? discuss its Components.
4) discuss Catabolite repression.
5) define operator & structural gene.


Q.5 ques. for six Marks
1) define the detail mechanism of Lac operon.
2) Comment on impotrance of Catabolite activator protein in lac operon
functioning.
3) discuss repression & induction mechanism of Lac operon.
4) define in detail regulation of Lac operon.

Earning:   Approval pending.