North Maharashtra University 2008 B.Sc Biotechnology S.Y.- - CELL BIOLOGY AND BASIC METABOLISM - Question Paper

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NORTH MAHARASHTRA UNIVERSITY, JALGAON

ques. Paper :- S.Y.BSc. - BIOTECHNOLOGY

BT 211 & 212 2008-09


CELL BIOLOGY AND BASIC METABOLISM
SEMESTER- FIRST
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Q.1 ques. for two Marks (Objective).
1) Which of the enzyme is involved in Transcription.
a) DNA Polymerase b) RNA Polymerase
c) Amino acyl tRNA transferase d) both b and c
2) …………..RNA is exist in high amount in cell.
a) t-RNA b) m RNA
c) r RNA d) none.
3) Thymine in DNA is changed by ……… nucleiotide in RNA.
a) Guanine b) Cytosine
c) Uracil d) Adenine.
4) ……………is the starting codon in translation.
a) AUG b) UAG
c) UGA d) UAA.
5) ……….is the 1st amino acid in proteins of bacteria.
a) N formyl methionine b) alanine
c) glycine d) Leucine.
6) 70S ribosome of bacteria are made up of……and……….subunits.
a) 50s and 30s b) 60s and 40s
c) 40s and 30s d) 60s and 10s
7) Activation of amino acid is carried out by ………… enzyme.
a) DNA Polymerase b) Amino acyle tRNA synthatase
c) peptidyl transferase d) both band c.
8) EF Tu and EF Ts complex of proteins are involved in ……………step of
translation.
a) Initiation b) Elongation
c) Activation d) Termination.
9) IF 150 ribonucleotides are current on mRNA, hence……….. number
of amino acids on the protein.
a) 75 b) 50
c) 100 d) 150.

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10) In eukaryotes ……… enzyme involved in synthesis of mRNA in
transcription.
a) RNA pol one b) RNA pol2
c) RNA pol three d) none.
Q.2 ques. for two Marks (Short answer).
1) describe transcription and what is the role of sigma factor.
2) describe translation and add a note on initiation and termination codon.
3) What is the role of RNA polymerase?
4) Draw the structure of mRNA and discuss it.
5) discuss 80s ribosome of eukaryotes.
6) Write a note on initiation of translation.
7) describe transcription and translation.
8) Write a short note on 70s ribosome.
Q.3 ques. for three Marks
1) discuss the structure of RNA.
2) Write a short note on: a)Initiation codon b)termination codon.
3) What is the role of t-RNA in translation?
4) Write the short note on structure of ribosome.
5) Distinguish ranging from 70s and 80s ribosome.
Q.4 ques. for four Marks
1) How protein elongation take place in translation?
2) define the process of translation termination?
3) Write a note on activation of amino acids.
4) discuss the difference ranging from transcription and translation.
5) discuss the process of transcription.
6) discuss the structure of 70s and 80s ribosome.
7) discuss the role of a) IF1 b) IF2 c) IF three d) EF Tu & EF Ts e) RF
8) Distinguish ranging from transcription and translation.
Q.5 ques. for six Marks
1) discuss the various properties of genetic code.

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2) discuss the function of tRNA ,mRNA and rRNA.
3) provide an account of elongation process of translation.
4) Write a note on transcription.

Earning:   Approval pending.