North Maharashtra University 2008 B.Sc Biotechnology S.Y.- CELL BIOLOGY AND BASIC METABOLISM - Question Paper

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NORTH MAHARASHTRA UNIVERSITY, JALGAON

ques. Paper :- S.Y.BSc. - BIOTECHNOLOGY

BT 211 & 212 2008-09


CELL BIOLOGY AND BASIC METABOLISM
SEMESTER- FIRST
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Q.1 ques. for two Marks (Objective).
1) ………….. enzyme relaxes the supercolling of double stranded DNA
molecule
a) DNA Polymerase b) Topoisoemerase
c) DNA helicase d) DNA Ligase
2) The agents that are responsible for bringing variation in genetic
message, known as ………………….
a) Unusual bases b) Tautomers
c) Mutagen d) Isomers
3) DNA polymerase – I enzyme was isolated by …………………
a) Beadle b) Tatum
c) Kornberg d) Edman
4) …………… react with bases that contain amino group for mutation.
a) Nitrous Acid b) Nitric Acid
c) Acetic Acid d) Ammonium Nitrate
5) DNA photolyse enzyme absorbs light of wavelength ranging from
……… to ………. nm.
a) 100 to 300 b) 200 to 300
c) 350 to 450 d) 300 to 500
6) RNA primers are synthesized by a template independent enzyme …….
a) dna B protein b) dna G protein

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c) DNA polymerase-I d) Topoisomerases
7) Replacement of a purine residue by a pyrimidine residue, the effect
termed as …………………
a) Mutation b) Substitution mutation
c) Transversion d) Transition
8) DNA polymerase – III add about ………………… nucleotide / sec.
a) 1000 b) 100
c) 10000 d) 10
9) ………………… part of DNA polymerase–III enzyme confers
processivity
a) core enzyme b) clamp loader
c) both a & b d) clamp
10) In base excision repair ……………… enzyme removes unusual bases.
a) DNA glycolyses b) DNA photolyse
c) DNA ligase d) AP endonuclease
11) ……………… protein brings termination of DNA replication.
a) stop b) tus
c) single strand binding d) ter
12) ……………… is known as long patch repair.
a) Mismatch repair b) Nucleotide excision repair
c) Direct repair d) Base excision repair
Q.2 ques. for two marks every
1) describe leading & lagging strand.
2) discuss role of DNA helicase.
3) Comment on action on DNA ligase.
4) discuss termination event in DNA replication.
5) describe substitution mutation with sub kinds.
6) Draw a diagram of Okazaki fragment formation.
7) Comment on role of dnaA & dnaB protein in DNA replication.
8) discuss briefly process of Alkylation.
9) Comment on direct repair mechanism.
10) provide role of DNA glycolyses & AP endonuclease in base excision
repair.

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Q.3 ques. for three marks every
1) What is oxidative deamination? discuss it.
2) discuss activities of DNA polymerase - I
3) define Okazaki fragment formation.
4) Comment on Direct repair mechanism.
5) describe Mutation & mutagenic agent
6) Compare: Natural & Artificial mutation.
7) Write a note on structure of DNA polymerase - III
8) discuss the initiation event in prokaryotic DNA replication.
9) describe Chemical & Physical mutagen.
10) Add a note on DNA ligase.
11) Comment on alkylation as chemical mutation.
12) discuss role of DNA helicase & single strand binding protein in DNA
replication.
Q.4 ques. for four marks.
1) define formation of Okazaki fragment.
2) Comment on formation of pysimidine dimer
3) define base excision repair
4) Write a note on structure & function of DNA polymerase - I
5) What is mutation? define oxidative deamination.
6) discuss the role of primer & template in DNA replication.
7) Comment on mismatch repair
8) Add a note on Nucleotide excision repair.
Q.5 ques. for six marks.
1) Enlist the enzymes involved in prokaryotic DNA replication & comment
on their function.
2) Comment on initiation, elongation & termination events in prokaryotic
DNA replication.
3) define long patch repair mechanism.
4) What is physical mutagen? Comment on pyrimidine dimer formation.

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5) Distinguish ranging from DNA polymerase I & DNA polymerase – II.

Earning:   Approval pending.