North Maharashtra University 2008 B.Sc Biotechnology S.Y- CELL BIOLOGY AND BASIC METABOLISM - Question Paper

18) Induced fit theory was proposed in-----------year.
a) 1950 b) 1953
c) 1958 d) 1957
19) Koshlands model also discuss the action of-------------.
a) Reversible Inhibition b) Competitive inhibition
c) Non competitive inhibition d) Allosteric inhibition
20) Km stands for-------------constant.
a) Koshland Menten b) Michealis Menten
c) Khune Menten d) None of these
ques. for two Marks (Short Answer)
1) provide the industrial significance of Protease.
2) provide the industrial significance of Amylase.
3) elaborate Apoenzymes & coenzymes?
4) Enlist the functions of the enzymes?
5) What is Lineweaver Burk equation?
6) provide the graphical presentation of the enzyme concentration.
7) Importance of temperature in enzymology.
8) elaborate the Isoenzymes?
9) provide the functions of the enzyme in any biochemical reaction.
10) Graphically represent effect of the pH, Temperature.
12) What is Activator? discuss with suitable example.
13) What is Inhibitor? discuss with suitable example
14) describe ß-oxidation.
15) Comment on Decarboxylation process.
16) What is Transmethylation.
17) Enlist essential and non essential amino acid.
ques. for three Marks
1) Definition of enzymes and add a note on its kinds.
2) What is the optimum temperature and the optimum pH of an enzyme?
3) elaborate coenzymes? discuss their characteristics.
4) elaborate the activators?
5) What is non-competitive inhibition?
6) How enzyme activity affect by PH?
7) Enlist the Importance of lipase.
8) discuss Koshland’s model.
9) Comparison ranging from competitive & non competitive inhibitors.
10) How Substrate concentration affect on enzyme?
ques. for four Marks
1) provide the Michelis- Menten formula.
2) What is enzyme? Add a note on its nomenclature? discuss I.U.B.?
3) discuss the classification of the enzymes with examples?
4) discuss the mechanism of enzyme catalysis?
5) elaborate the significance of the enzyme concentration?
6) What is enzyme inhibition? State its kinds.
7) elaborate activators and inhibitors? discuss with suitable example.
8) Enlist the factors affecting enzyme activity.
9) State the applications of the proteases and the lipases.
10) What is Enzymology? discuss the concept of biocatalysts
11) What do you mean by essential amino acid? Add a note on
biosynthesis of any 1 amino acid.
12) discuss biosynthesis of Proline?
13) provide the regulatory role of enzyme in Gluconeogenesis.
14) discuss transamination reaction on protein.
ques. for six Marks
1) provide the classification of the enzymes with suitable examples
2) discuss the enzyme-substrate complex reaction.
3) What is optical specificity and the substrate specificity?
4) discuss feed back inhibition?
5) explain in detail ß-oxidation of fatty acid .
6) provide the reaction in which oxaloacetic acid is converted in to citric acid
7) explain in detail Gluconeogenesis.
8) discuss protein degradation in details.

Earning:   Approval pending.