National Institute of Technology 2006 B.Tech Chemical Engineering Biochemistry - Question Paper

t NATIONAL INSTITUTE OF TECHNOLOGY KARNATAKA, SURATHKAL, SRINIVASNAGAR
575 025 DK
Department of Chemistry
III SEM. B. TECH. (CH) Mid- Sem. Examination, October, 2006 Elective: Biochemistry (CY-302,3-0-0)
Time: 90 minutes. Max. marks: 40
Note: ans all ques.. every ques. carries eight marks.
1. (a) discuss (i) Endoplasmic reticulum & (ii) Golgi complex. Mention their functions
in the cell. (3 marks)
(b) A sample of microbial protein was obtained to contain 7.5 % nitrogen by Kjeldahl's
method. compute the percentage of protein current in it. (1 mark)
(c) Write the Haworth's structures of (i) alpha and beta Glucose (ii) Sucrose and
(iii) Chondroitin - four - sulphate. (2 marks)
(d) Explain briefly (i) Cellulose and (ii) Chitin. (2 marks)
2. (a) elaborate lipids ? How are they classified ? provide examples. (3 marks)
(b) Explain the structure and functions of Sphingomyelins (2 marks).
(c) Write the structure of cholesterol. Mention its properties and functions. (3 marks)
3. (a) explain the classifications of amino acids giving examples for every class. (4 marks)
(b) discuss how sequence of amino acids is determined using Sanger's reagent. Write briefly on a - helix structure of protein molecules. (4 marks)
4. (a) Compare the salient features of tertiary and quaternary structures of proteins.
(3 marks)
(b) Write an account of (i) Precipitation of proteins at pi & (ii) Biuret reactions of
proteins. (3 marks)
(c) What is denaturation of protein ? discuss the characteristics of denaturation.
(2 marks)
5. (a) What is Chargaff s rule of DNA composition ? discuss double helical structure of
DNA. (4 marks)
(b) Name the important bases and carbohydrates current in nucleic acids. Write their
structures. (2 marks)
(c) Give an example of biologically active peptide. Write any 3 functions of it.
(2 marks)
1
* * * + 4

Earning:   Approval pending.