Madras University (UnOM) 2006 B.Sc Biotechnology Application Oriented Subject II - Micro Technique - Question Paper

Application Oriented Subject II - Micro Technique

Time -3 Hrs
Maximum: 100 marks

part - A [10 x three =Marks 30]

ans any TEN ques..All ques. carry equal marks

every ans should not exceed 50 words. Draw diagrams wherever necessary.

1. Smear.
2. FAA.
3. Squash technique.
4. Mordant.
5. Infiltration.
6. Sledge Microtome.
7. Phase Contrast Microscopy.
8. Dehydration.
9. Uses of Condenser.
10. Vital stains.
11. Camoy's Fluid.
12 Micrometry.

part - B [5 x six = Marks 30]

ans any 5 ques. All ques. carry equal marks

every ans should not exceed 200 words.

13. State the advantages and limitations of Electron microscopy.

14. define the structure and function of a Rotary - Microtome.

15. What do you mean by fixation? provide 2 examples.

16. What is embedding? How is it carried out?

17. discuss the whole mount preparation of algae.

18. define Feulgen's reaction and Feulgen stain.

19. discuss the procedure to stain Bacteria.

part - C [4 x 10 = Marks 40]

ans any 4 ques. All ques. carry equal marks

every ans should not exceed 500 words

20. Trace the pathway of light in a light microscope.

21. Write in detail about preparation of part by praffin method.

22. provide a detailed procedure for sectioning and staining of an angiosperm method by using microtome.

23. define the procedure and chemicals used for maceration.

24. Write the different kinds of Ocular and objective lenses and add a note on its uses.

25. explain the merits and demerits of different microtomes learned by you.

Earning:   Approval pending.