Madras University (UnOM) 2003 B.Sc Biochemistry - Question Paper

OCTOBER 2003 13694/DJE
Time : 3 hours Maximum : 100 Marks


part A – (10 X three = 30 marks)

ans ALL ques.

All ques. carry equal marks


1. Explain perfusion technique.
2. Name any 3 compounds added to media to maintain the sulfhydryl grou of a protein during separation.
3. What is glycogenin? explain its role.
4. Write a note on sialic acid and its importance.
5. Discuss the synthesis of galactosamine.
6. Bring out the enzymes bound to inner membranes and matrix of mitochondria.
7. Oxidation of a fatty acid with an odd number of carbon atoms are glucogenic. discuss.
8. Describe the pathways of ketogenesis in the liver.
9. How will you synthesize phosphoadenosine phosphosulfate?
10. Explain glutamate dehydrogenase reaction and its significance.


part B – (5 X six = 30 marks)

ans any 5 ques.

All ques. carry equal marks


11. Comment on the media for tissue homogenization and separation.
12. Write a note on the hormonal regulation of glycogen metabolism.
13. Illustrate the reactions catalysed by pyruvate dehydrogenase complex.
14. Bring out the 4 complexes of electron transport chain.
15. Describe the conversion of linoleate to arachidonate.
16. Explain a–oxidation of fatty acids and its importance.
17. Describe deamination and transamination with examples.

part C – (2 X 20 = 40 marks)

ans BOTH the ques.

BOTH ques. carry equal marks

18. (a) explain in detail the TCA cycle, its amphibo nature and regulation.
Or
(b) Outline the structure of mitochondr membranes and explain the principles of the chemiosmotic theory of oxidative phosphorylation.


19. (a) (i) define the fatty acid synthet complex and biosynthesis of fatty acid.
(ii) How is biosynthesis of fatty regulated?
Or
(b) what are the biosynthesis and degradation haem.

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Earning:   Approval pending.