Madras University (UnOM) 2001 B.Sc Biochemistry - Question Paper

APRIL 2001 13694/DJE
Time : 3 hours Maximum : 100 Marks


part A – (10 X three = 30 marks)

ans ALL ques.

All ques. carry equal marks


1. How will you prepare tissues slices? And provide its application.
2. What are marker enzymes? provide its uses.
3. How is flow of pyruvate into citric acid cycle regulated by pyruvate dehydrogenase complex?
4. Explain the importance of NADPH generated in pentose phosphate pathway.
5. Give the importance of phosphocreatine along with its structure.
6. What are unicouplers? Name 3 of them.
7. What are ketone bodies? How are they formed?
8. Explain a –oxidation of fatty acids.
9. Explain a reaction in which PAPS acts as a substrate.
10. What are the steps involved in the formation of serotonin? discuss.


part B – (5 X six = 30 marks)

ans any 5 ques.

All ques. carry equal marks


11. Explain uronic acid pathway and add a note on the importance of glucuronic acid.
12. Explain glyoxalate cycle.
13. Describe the chemiosmotic theory.
14. How is xenobiotics detoxified?
15. How is phenylalanine degraded?
16. Explain the step involved in the synthesis of cephalin.
17. How does urea cycle operate in the liver?

part C – (2 X 20 = 40 marks)

ans BOTH the ques.

BOTH ques. carry equal marks

18. (a) How will you prepare a tissue homogenate? discuss the steps involved in fractionation of a tissue homogenate. How will you use the fractionated organelles to study metabolic compartmentalisation?
Or
(b) (i) How fatty acids are degraded? (10)
(ii) How does epinephrine control glycogen metabolism? (10)


19. (a) (i) How is heme synthesized? (10)
(ii) discuss the stepwise synthesis of various prostaglandin (10)
Or
(b) Write notes on:

(i) Phospholipases
(ii) Bioluminescence
(iii) Substrate level oxidative phosphorylation
(iv) Glycoprotein

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Earning:   Approval pending.