Madras University (UnOM) 2002 B.Sc Biochemistry - Question Paper

APRIL 2002 13694/DJE
Time : 3 hours Maximum : 100 Marks


part A – (10 X three = 30 marks)

ans ALL ques.

All ques. carry equal marks


1. How will you employ a whole organism to study a biochemical reaction?
2. What are marker enzymes?
3. Give the exportance of hexose monophosphate shunt.
4. How is pyruvate metabolised through TCA cycle if malonate is added in the incubation medium.
5. Explain bioluminescence.
6. Oxidation of NADH through ETC produced three ATP, while FADH2 produces only 2. Why?
7. What are ketone bodies?
8. Give the structure of cholic acid and its importance.
9. What are ketogenic and glycogenic amino acids? provide examples.
10. What are sulphatases?


part B – (5 X six = 30 marks)

ans any 5 ques.

All ques. carry equal marks


11. Explain the steps involved in producing cells in culture.
12. How is glucose metabolised through HMP shunt?
13. How is pyruvate converted to acetyl CoA?
14. Explain glyoxylate cycle.
15. What are prostaglandins? How are they synthesised?
16. How is fatty acid oxidised in the mitochondria?
17. Explain the urea cycle.

part C – (2 X 20 = 40 marks)

ans BOTH the ques.

BOTH ques. carry equal marks

18. (a) discuss in details how epinephrine regulates glycogen metabolism. Add a note on phosphofructokinase regulation.
Or
(b) What is a multi enzyme complex? How is a fatty acid synthesised? discuss the steps in detail


19. (a) discuss in detail the steps involved in oxidative phosphorylation and chemiosmotic theory. Add a note on uncouplers.
Or
(b) Write notes on:

(i) Detoxication
(ii) Phospholipids
(iii) Pyridoxal phosphate
(iv) Glycoprotein

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Earning:   Approval pending.