Madras University (UnOM) 2003 B.Sc Biochemistry bio chemistry - Question Paper

APRIL 2003 13694/DJE
Time : 3 hours Maximum : 100 Marks


part A – (10 X three = 30 marks)

ans ALL ques.

All ques. carry equal marks


1. Write a note on slice technique.
2. What is meant by in vitro studies?
3. How is fructose-1,6 biophosphatase enzyme regulated?
4. Why is TCA cycle called amphibolic pathway?
5. Explain bioluminescence with an example.
6. Name any 2 chemical uncouplers of oxidative phosphorylation.
7. How does long chain acyl-CoA pass through the inner mitochondrial membrane?
8. Give an example for ‘suicide enzyme’.
9. Write the catabolism of cysteine by transamination pathway.
10. List out glucogenic and ketogenic amino acids.


part B – (5 X six = 30 marks)

ans any 5 ques.

All ques. carry equal marks


11. Give a scheme for the fractionation of rat liver homogenate into different subcellular fractions.
12. Narrate glyoxalate cycle and explain the importance of that cycle.
13. Describe the synthesis of ascorbic acid.
14. Oxidation of extramitochondrial NADH is mediated by substrate shuttles. discuss.
15. Explain the synthesis of bile acids.
16. Bring out the biosynthesis of sphingomyelin.
17. Write a note on the biosynthesis of cysteine from homocysteine and serine.

part C – (2 X 20 = 40 marks)

ans BOTH the ques.

BOTH ques. carry equal marks

18. (a) (i) explain in detail gluconeogenesis.
(ii) Describe the regulation of glycolysis and gluconeogenesis.
Or
(b) Bring out phasel and phase2 reactions of xenobiotic metabolism.


19. (a) provide an account on the biosynthesis of prostagland thromboxanes, leukotrines and hydroxyeicosanoic acids.
Or
(b) what are the metabolism of heam.

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Earning:   Approval pending.