National Institute of Technology 2008 B.Tech Chemical Engineering BIOCHEMISTRY - Question Paper

ans all ques..
every ques. carries eight marks.


1. [a] discuss Endoplasmic reticulum & [ii] Golgi complex. Mention their functions
in the cell. [3 marks]

A sample of microbial protein was obtained to contain 7.5 % nitrogen by Kjeldahl's
method. compute the percentage of protein current in it. [1 mark]

Write the Haworth's structures of alpha and beta Glucose [ii] Sucrose and

[iii] Chondroitin - four - sulphate. [2 marks]

discuss briefly Cellulose and [ii] Chitin. [2 marks]


2. [a] elaborate lipids ? How are they classified ? provide examples. [3 marks]

discuss the structure and functions of Sphingomyelins [2 marks].

Write the structure of cholesterol. Mention its properties and functions. [3 marks]


3. [a] explain the classifications of amino acids giving examples for every class. [4 marks]

discuss how sequence of amino acids is determined using Sanger's reagent. Write briefly on a - helix structure of protein molecules. [4 marks]


4. [a] Compare the salient features of tertiary and quaternary structures of proteins.
[3 marks]

Write an account of Precipitation of proteins at pi & [ii] Biuret reactions of
proteins. [3 marks]

What is denaturation of protein ? discuss the characteristics of denaturation.
[2 marks]


5. [a] What is Chargaff s rule of DNA composition ? discuss double helical structure of
DNA. [4 marks]

Name the important bases and carbohydrates current in nucleic acids. Write their
structures. [2 marks]

provide an example of biologically active peptide. Write any 3 functions of it.
[2 marks]

Earning:   Approval pending.