Jawaharlal Nehru Technological University Kakinada 2010-1st Sem M.Tech Biotechnology SUBJECT CODE: C0303 REGULAR S BIO CHEMICAL ENGINEERING - Question Paper

1. Derive the Michealis-Menten formula by using
a) The rapid equilibrium assumption and
b) quasi-steady state assumption
for simple enzyme kinetics that involves a reversible step for enzyme-substrate
complex and a dissociation step of the ES complex.

2. Write in detail about active and passive immobilization of cells.

3. Aerobic degradation of benzoic acid by a mixed culture of microorganisms can
be represented by the subsequent reaction.

C6H5COOH + a O2 + b NH3
(Substrate)

c C5H7NO2 + d H2O + e CO2
(Bacteria)

a.Determine a, b, c, d, and e if RQ = 0.9
b.Determine the yield coefficients, YX/S and YX/O2
c.Determine degree of reduction for the substrate and bacteria.


4. provide a brief account of packed bed bioreactors and trickle bed bioreactors.

5. a) explain in detail about thermal death kinetics.
b) discuss the process of sterilization of air.

6. provide a detailed account of alternate bioreactor configurations.

7. a) describe simple and complex media. provide an example for every.
b) explain about medium requirements and medium formulation.

8. With respect to mass transfer into solid particles, write about
a) External mass transfer resistance
b) Intra-particle diffusion and reaction

Earning:   Approval pending.