Jawaharlal Nehru Technological University Kakinada 2010-1st Sem M.Tech Biotechnology SUBJECT CODE: C0305 REGULAR S BIO ANALYTICAL TECHNIQUES - university paper

1. describe the subsequent terms
(a)Mobile Phase
(b)Retention factor
(c)Elution analysis
(d)Selectivity factor


2. (a) define the principle of size exclusion Chromatography?
(b) What is a molecular sieve?


3. (a) What is meant by Retention time and the Rf value?
(b) What is the Significance of t0 in gas chromatography?


4. Write the importance of Reverse Phase HPLC and also write its applications?


5. (a) What is the finger print region and how it is used?
(b) What is Hydrogen bonding? How does it affect the IR frequencies?


6. discuss the subsequent terms and effects
(a) Shielding(b) De-shielding (c) Chemical Shift

7. (a) compute the PH of a solution prepared by mixing 2ml of a strong acid solution of PH =3
and 3ml of a strong base of PH =10
(b) discuss Buffer capacity?
(c) discuss the most common methods of measuring pH and write the relation ranging from
composition and pH


8. (a) Write a short notes on Ultra filtration with emphasis on its application in analytical
chemistry?
(b) Differentiate ranging from dialysis and centrifugal dialysis

Earning:   Approval pending.