Gujarat Technological University 2010 Master in Pharm (Ayu.) Quality Assurance G.T.UM.pharm Modern Analytical Techniques - Question Paper
G.T.U. M.pharm Modern Analytical Techniques
Seat No.: Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
M. Pharmacy Sem-I Examination January 2010 Subject code: 910001 Subject Name: Modern Analytical Techniques Date: 19 / 01/ 2010 Time: 12.00 - 3.00 pm
Instructions: Total Marks: 80
1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1 (a) Explain the term resolution, column selectivity and capacity factor. 08 Describe the options for changing the selectivity a.
(b) Explain the principle of size exclusion chromatography. Describe 08 stationary phases used in SEC.
Q.2 (a) Enlist the factors affecting the efficiency of chromatographic 06 separation. Discuss longitudinal diffusion.
(b) Describe chemical ionization technique with its advantages and 05 disadvantages.
(c) Give chemical shift values and spin-spin splitting for the following 05 compounds:
i. Benzyl acetate ii. Ethanol iii. Ethyl methyl ether
Q.3 (a) What is plasma? Discuss inductively coupled plasma emission 06 spectroscopy.
(b) What is enzyme immune-assay? Describe double sandwich ELISA 05 technique for antigen measurement.
(c) How will you differentiate o, m and p xylene on the basis of their 05 proton decoupled CMR spectrum.
Q.4 (a) Describe the factors affecting the chemical shift. 06
(c) Describe theory and application of derivative spectroscopy. 05
Q.5 (a) Explain the following statements: 06
i. Maldi is used to determine the molecular weight of proteins.
ii. Anilinium cation exhibits UV spectrum almost similar to benzene.
iii. C 13 NMR spectra are more difficult to record than PMR
iv. The value of capacity factor k should be between 1 to 10.
(c) Describe with diagram, principle and working of Michelson 05 interferometer.
Write short notes on the following:
Q. 6
16
a. HPTLC
b. Isoelectric focusing
c. Mc-lafferty rearrangement
Q.7 (a) Describe technique of Affinity chromatography.
06
(b) Identify the following compounds on the basis of the spectral data 10 presented here. Show your reasoning for the conclusion arrived at.
i. UV: 265 nm ( e = 450)
IR: 3330, 2970, 2880, 1515, 1465, 813 cm-1 NMR: (5) 1.12 d (6H) J=7.0 Hz 2.28 s (3H)
2.82 heptate (1H) J=7.0 Hz
7.02 s (4H)
CMR: 21.3, 24.2, 38.9, 126.6, 134.8, 145.7 MS: M+ 134, 119, 77.
ii. UV: Not more than 210 IR: 2980, 2800, 2170, 1745, 1200 cm-1 NMR: (5) 1.3 t (3H)
3.5 s (2H)
4.3 q (2H)
MS: 113(M+\ 86, 68, 67.
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| Earning: Approval pending. |
