Gujarat Technological University 2010 M.Pharm Modern Analytical Techniques - Question Paper

Q.1 (a) discuss the term resolution, column selectivity and capacity factor.
define the choices for changing the selectivity
.
08
(b) discuss the principle of size exclusion chromatography. define
stationary phases used in SEC.
08
Q.2 (a) Enlist the factors affecting the efficiency of chromatographic
separation. explain longitudinal diffusion.
06
(b) define chemical ionization technique with its advantages and
disadvantages.
05
(c) provide chemical shift values and spin-spin splitting for the subsequent
compounds:
i. Benzyl acetate ii. Ethanol iii. Ethyl methyl ether
05
Q.3 (a) What is plasma? explain inductively coupled plasma emission
spectroscopy.
06
(b) What is enzyme immune-assay? define double sandwich ELISA
technique for antigen measurement.
05
(c) How will you differentiate o, m and p xylene on the basis of their
proton decoupled CMR spectrum.
05
Q.4 (a) define the factors affecting the chemical shift. 06
(b) define methods used for simplification of complex spectra. 05
(c) define theory and application of derivative spectroscopy. 05
Q.5 (a) discuss the subsequent statements:
i. Maldi is used to determine the molecular weight of proteins.
ii. Anilinium cation exhibits UV spectrum almost similar to
benzene.
iii. C 13 NMR spectra are more difficult to record than PMR
iv. The value of capacity factor k' should be ranging from one to 10.
06
(b) define storage, handling and documentation of reference standard. 05
(c) define with diagram, principle and working of Michelson
interferometer.
05
Q. six Write short notes on the following:
a. HPTLC
b. Isoelectric focusing
c. Mc-lafferty rearrangement
16
Q.7 (a) define technique of Affinity chromatography. 06

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Earning:   Approval pending.