Gujarat Technological University 2010-5th Sem B.Pharm BPharmacy Pharmaceutical Microbiology- I - Question Paper

Seat No.: _____ Enrolment No.______
GUJARAT TECHNOLOGICAL UNIVERSITY
B. Pharmacy Sem- Vth exam December 2010
Subject code: 250002
Subject Name: Pharmaceutical Microbiology- I
Date: 16 /12 /2010 Time: 02.30 pm – 05.30 pm
Instructions: Total Marks: 80
1. Attempt any 5 ques..
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1 (a) describe pharmaceutical microbiology. define its scope in brief. 06
(b) define contribution of 'Louis Pasteur' in microbiology. 05
(c) Write note on bacterial flagella. 05
Q.2 (a) Explain: Motility, Pathogenicity, Virulence 06
(b) define kind of bacteria on the basis of :
i) Nutritional requirement ii) Temperature requirement
05
(c) Classify staining techniques. explain gram staining. 05
Q.3 (a) Enlist methods for isolation of bacteria. explain streak plate method. 06
(b) Write note on Scanning Electron Microscopy 05
(c) define factors affecting disinfection process in brief 05
Q.4 (a) Enumerate methods for valuation of disinfectants. define Rideal-Walker test. 06
(b) Explain: Thermal death point, Thermal death time, Iodophore, Bioburden,
Bactericide
05
(c) describe 'sterilization'. Classify various methods for the identical with mechanism of
action.
05
Q.5 (a) What is cold sterilization? Write note on fractional sterilization. 06
(b) Explain: D- value, F- value, Z- value, Q10- value, Inactivation factor 05
(c) define biological indicators for monitoring sterilization processes 05
Q. six (a) Enlist methods for sterility testing. explain direct inoculation method. 06
(b) Explain: Sterility, SAL, Incineration, MIC, Zone of inhibition 05
(c) define cup-plate method for microbiological assay 05
Q.7 (a) discuss microbiological assay. Name microbes used in assay of: Amphotericin B,
Erythromycin, Carbenicillin, Rifampicin, Tetracycline
06
(b) explain microbiological assay of cyanocobalamin 05
(c) Explain: Total count & Viable count. define photometric method for determining
total count
05

Attachment: gujarat-technological-university-2010-b-pharm-52723-1.zip

Earning:   Approval pending.