Osmania University (OU) 2009-1st Year B.Pharm B pharmacy second semester microbiology osmania (2.1) - Question Paper

1. a) i) Write about preservation of pure cultures.
ii) Write about transmission electron microscopy.

OR

b) i) explain different methods for isolation of pure cultures from a mixed culture.
ii) Write about selective and differential media.

2. a) i) define the morphology and classification of Actinomycetes
ii)
describe differential staining with examples. Differentiate ranging from gram-positive and gram-negative bacteria.

OR

b) i) With the help of a neat diagram, define the structure of a typical bacterial cell.
ii) Write a note on acid-fast staining.

3. a) i) explain sterilisation by gamma rays and gases.
ii) Write about the advantages of sterilisation by filtration method.

OR

b) i) explain different factors influencing disinfection.
ii) elaborate the measures to be taken to maintain aseptic environment in the microbiology laboratory?

4. a) i) describe immunity and classify immunity with examples and explain the principles of various kinds pf immunity.
ii) What is meant by cell mediated immunity? How is it imparted?

OR

b) i) Distinguish ranging from endotoxins and exotoxins.
ii) Write about primary and specific defensive mechanism of body.

5. a) i) discuss the general scheme for the detection of coliforms in water samples.
ii) discuss the mode of transmission ans symptoms of the subsequent diseases
A)Typhoid B) Diphtheria

OR

b) i) Write a note on the principle, procedure and application of pasteurisation.
ii) discuss the mode of transmission and symptoms of the subsequent diseases:
A) Hepatitis-A B) Malaria

Earning:   Approval pending.