Jawaharlal Nehru Technological University Hyderabad 2010-1st Sem M.Tech Biotechnology Regular s ch/il ENZYME ENGINEERING & TECHNOLOGY ( ) - Question Paper

Code No: D109110310
JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD
M.Tech I Semester Regular exams March/April 2010
ENZYME ENGINEERING & TECHNOLOGY
(Biotechnology )
Time: 3hours Max.Marks:60
ans any 5 ques.
All ques. carry equal marks
- - -
1. explain different graphical methods for estimation of M.M. constants?
2. What is enzyme inhibition? explain the kinetics of competitive inhibition?
3. explain the kinetic model to show the effect of temperature on enzyme kinetics?
4. What is Immobilization? explain different immobilization methods?
5. Write a note on External & internal mass transfer resistance in immobilized
Enzymes?
6. What is Briggs & Haldane approach? How is it various from Michaelis Menten
Approach?
7. explain the kinetics of multisubstrate enzyme catalysed reactions?
8. explain the subsequent a) Damkohler Number (4)
b) Turn over number (4)
c) Specific activity (4)

Earning:   Approval pending.