Jawaharlal Nehru Technological University Hyderabad 2010-2nd Sem M.Tech Biotechnology Regular s tember, GENETIC ENGINEERING - Question Paper

Code No: B0302/D0302
JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD
M.Tech II - Semester Regular exams September, 2010
GENETIC ENGINEERING
(Biotechnology)
Time: 3hours Max. Marks: 60
ans any 5 ques.
All ques. carry equal marks
- - -

1) Write a brief notes on
a) DNA sequencing and its importance
b) various kinds of mutation

2) discuss
a) How DNA molecules can be ligated without DNA ligase? discuss the
process involved
b) How G+C content of a DNA molecule affects its susceptibility to various
restriction endonuclease?

3) discuss how a genome library is constructed and utilized for screening of
transformants?

4) discuss the technique gene shuffling with an example.

5) explain the subsequent
a) Challenges in find full length cDNA for cloning with an example.
b) How PCR can be used as an option for the screening of genomic and
cDNA libraries?

6) discuss the methods involved in transcript analysis.

7) Compare and contrast ranging from the methods of attaining expression in bacteria and
yeast.

8) discuss the methods utilized towards getting a useful gene replacement in animal
model.

Earning:   Approval pending.