Jawaharlal Nehru Technological University Hyderabad 2010-2nd Sem M.Tech Biotechnology Regular s, - Question Paper

Code No: D0308 RO9
JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD
M.Tech II - Semester Regular Examinations, October 2010
ADVANCED ANIMAL CELL SCIENCE AND TECHNOLOGY
(BIOTECHNOLOGY)
Time: 3hours Max. Marks: 60
ans any 5 ques.
All ques. carry equal marks
- - -
1) explain in detail about different physico-chemical properties attributed to the
culture medium by the components of the medium.

2) discuss about the role of oncogenes and the mechanisms involved in
immortalization process of a cell.

3) explain the subsequent
a) Transformation by using viruses.
b) Role of telomerase in cancer detection.

4) explain in detail about the cloning procedure employed for obtaining a monolayer
of clones.

5) describe the term cell synchronization. explain about the methods involved.

6) discuss RNA silencing and explain the role of RNA silencing in gene knockout
technique.

7) explain the different steps involved in somatic cell fusion? Write short notes on its
application.

8) Using a gene related genetic defect in human as a hypothetical example, discuss
how to gene knockout technology be put to use in gene therapy?


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Earning:   Approval pending.