University of Hyderabad (UoH) 2010 Entrance Exams Other Entrance Exams M.Sc Programme Entrance - Molecular Microbiology - Question Paper

C.    2 mM

D.    4mM

24.    An antibiotic should inhibit or kill the pathogen while harming host as little as possible. In this context, an antibiotic should focus on a microbial target not found in mammalian cells. By this standard, which of the following antibiotic agent would be expected to be most toxic to humans?

A.    Cephalosporin

B.    Mitomycin

C.    Penicillin

D.    Bacitracin

25.    Identify the mismatch

A.    Escherichia- MR+; VP-; Indole+

B.    Enterobacter-MR-; VP+; Indole-

C.    Klebsiella pneumoniae-MR-; VP+; Indole

D.    Escherichia-MR--, VP+; Indole+

26.    Monotropa (Indian pipe plant) is

A.    a plant that has nodules formed by an actinomycete

B.    a plant that has nodules formed by Rhizobium

C.    a non-photosynthetic plant that obtains its carbohydrates by mycorrhizal fungi

D.    a non-photosynthetic plant that obtains its amino acids by mycorrhizal fungi

27.    Actinorhizal plants

A.    have higher content of actin than other plants

B.    lack actin filaments

C.    may have nitrogen-fixing root nodules formed by Rhizobium

D.    include the red alder and Casuarina

28.    Mycorrhizal roots contain symbiotic organism(s) which help primarily in the process of

A.    lateral (branch) root formation

B.    nitrogen fixation

C.    uptake of phosphorus and water

D.    nodule formation

29.    The most common phycobiont in lichens is

A.    red alga

B.    brown alga

C.    blue-green alga

D.    green alga

30.    A fungus parasiting another fungus is called

A.    epiphyte

B.    epibiotic

C.    parasite

D.    mycoparasite

31.    The living fossil Ginko biloba is

A.    Angiosperm

B.    Gymnosperm

C.    Pteridophyte

D.    Moss

32.    The organism spirogyra is so named because of the nature of

A.    Chloroplast

B.    Cell wall

C.    Plasma membrane

D.    Pyrenoid

33.    Pneumatophores are associated with

A. Xerophytes

r> a a__;________

l>. mangiuuvca

C.    Lithophytes

D.    Hydrophytes

^M butladct flowers?^l0Win8 W^{Uld yU plaCC} 3 plant which P^roduces seeds

A.    Angiosperm

B.    Pteridophytes

C.    Bryophytes

D.    Gymnosperm

35.    How many ATP and reducing equivalents required to synthesize one molecule of hexose by Calvin cycle in C3 plants?

A.    3 ATP and 2 NADPH

B.    12 ATP and 18 NADPH

C.    18 ATP and 12 NADPH

D.    36 ATP and 18 NADPH

36.    The core of nucleosome structure is due to

A.    non-histone proteins

B.    Histone H2A, H2B, H3 and H4

C.    Histone H2A, H2B, H3A, H3B and H4

D.    Nucleoid proteins

37.    Sargassum is a genus of brown macroalga in the order Fucales which comes in the class of

A.    Rhodophyceae

B.    Phaeophyceae

C.    Raphidophyceae

D.    Chrysophyceae

38.    People generally enjoy picking mushrooms when they see them in the wild. However, there are quite a number of very dangerous wild mushrooms, for example some in the genus called Amanita. Which of the following is NOT a distinguishing characteristic of the genus Amanita?

A.    blue-colored rings

B.    cup at its base    ,

C.    White gills

D.    Ring-stem

39.    The synthesis of starch and sucrose in plants uses__: as the substrate,

rather than_, which is used in the synthesis of glycogen in animal cells.

A.    ADP-fructose; UDP-glucose

B.    ADP-glucose; UDP-glucose

C.    fructose 1-phosphate; glucose 1-phosphate

D.    UDP-glucose; ADP-glucose

40.    In a cell, the number of chromosomes is 24 after first meiosis. The number of chromosomes in its daughter cells after completion of meiosis will be

A.    24

B.    12

C.    48

D.    06

41.    What part of lipid molecule is in the middle of a lipid bilayer of the cell membrane?

A.    Glycerol

B.    Phosphate group

C.    Fatty acid

D.    Amino Acid

42. Cells can be described as having a cytoskeleton of internal structures that contribute to the shape, organization and movement of the cell. All of the following are part of the cytoskeleton except

A.    the cell wall

B.    microtubules

C.    actin filaments

D.    intermediate filaments

43.    Which of the following pairs of genera are members of the zygomycetes class and can be seen on microscopic examination to possess rhizoids?

A.    Rhizopus and Absidia

B.    Rhizopus and Mucor

C.    Cladosporium and Rhizopus

D.    Cladosporium and Absidia

44.    The phenomenon of exudation of water from the leaves during cool nights is called

A.    transpiration

B.    translocation

C.    guttation

D.    secretion

45.    Schizogony and schizont stage in the life history of malaria parasite occur in

A.    RBCs and liver of man

B.    salivary gland of male anopheles

C.    salivary gland of female anopheles

D.    stomach of female anopheles

46.    Cartilage is produced by

A.    osteoblast

B.    chondrocytes

C.    melanoblasts

D.    fibrocytes

47.    Most hormones, particularly those released by the hypothalamus and pituitary, fall into which of the following chemical class

A.    Amines

B.    Glycoproteins

C.    Peptide hormones

D.    Steroids

48.    A gene inherited from a female is not expressed in either male or female offspring, but the same gene is expressed in both male and female offspring if inherited from a male. This phenomenon is an illustration of gene

A.    recombination

B.    repression

C.    deletion

D.    imprinting

49.    Which of the following hormone controls the mood, appetite and sleep?

A.    Calcitonin

B.    Erythropoietin

C.    Serotonin

D.    Gastrin

50.    Which of the following cellular activity is NOT promoted by insulin?

A.    the storage of glycogen by the liver

B.    the storage of glycogen by muscles

C.    the storage of fats by adipose tissue (fat cells)

D.    the creation of glucose from amino acids

51.    The organism Enterobius vermicularis belongs to order

A.    Kinetoplastidae

B.    Oxyurida

C.    Strigeidida

D.    Ascaridida

52.    Which of the following processes leads to formation of polytene chromosomes in salivary gland chromosomes of Drosophila?

A.    Repeated replication without separation of chromatids

B.    Repeated replication followed by separation of chromatids

C.    Nondisjunction of chromatids during meiosis

D.    Nondisjunction of chromatids during mitosis    

53.    The mode of action of a steroid hormone involves:

A.    binding to a cell membrane receptor

B.    activation of protein kinases

C.    binding to calmodulin

D.    modifying gene transcription

54.    Which of the following most closely resembles the mammals, sharing with them several derived features of the skull and teeth?

A.    Pelycosaurs

B.    Trihelodontids

C.    Cynodonts

D.    Polyphyodonts

55.    Highly developed organisms are made up of many different cell types. In order for the organism to function properly, the cells must work together. This is accomplished by various types of chemicals that act as messengers to the cells. Each chemical messenger has a specific effect on its target cell. This is called signal transduction. Hormones are good examples of chemical messengers. Steroid hormones such as estrogen are non-polar and can thus diffuse across the cell membrane. However, polar hormones cannot. For their message to enter the cell these polar hormones must

A.    form a micelle

B.    bind to receptors on the cell surface

C.    bind to G proteins

D.    be chemically altered

56.    Giardia is

A.    a plant that has nodules formed by an actinomycete

B.    a non-photosynthetic plant that acquires its carbohydrates by mycorrhizal fungi

C.    an eukaryote that lacks mitochondria and causes Beaver fever

D.    a prokaryote that contain both DNA and RNA and causes Beaver fever

57.    During acute, non-specific inflammation, the first leucocytes to arrive at the site of tissue damage are

A.    monocytes

B.    lymphocytes

C.    macrophages

D.    neutrophils

58.    A person does not normally produce antibodies or activated T cells to self antigen due to the presence of

A.    haptens

B.    immunoglobulins

C.    human leucocyte antigen

D.    non-self antigen

59.    Which of the following drug is used as antihelminthic for the treatment of ascariasis? This drug causes flaccid paralysis of ascaris by blocking acetylcholine at the myoneural junction.

A.    Piperazine

B.    Diloxanide

C.    Mefloquine

D.    Flucytosine

60.    Which of the following interleukins enhances the type I hypersensitivity reaction

A.    IL-1

B.    IL-2

C.    IL-3

D.    IL-4

61.    Who proposed the theory stating that cell emerges from the pre-existing cells by cell division

A.    Ernst Ruska

B.    Rudolph Virchow

C.    Robert Hooke

D.    Robert Brown

62.    The RNA virus having DNA as an intermediate in its life cycle is

A.    Human immunodeficiency virus (HIV)

B.    Hepatitis C virus (HCV)

C.    Hepatitis B virus (HBV)

D.    None

63.    The hinge region of an Immunoglobin IgG heavy chain is located

A.    between V_H and Chi

B.    between Ch2 and Ch3

C.    between Chi and Cm

D.    within the Fc fragment

64.    When ethylene is treated with sulphur monochloride, a toxic product is obtained, which is

A.    Tear gas    ,

B.    Thiophene

C.    Mustard Gas

D.    Dimethyl sulphoxide (DMSO)

65.    When an unsymmetrical reagent adds to an unsymmetrical double bond the positive part of the reagent becomes attached to the double bounded carbon atom which bears the greatest number of H-atoms. This rule is popularly known as

A.    Huckel rule

B.    Saytzeff rule

C.    Fischer rule

D.    Markovnikov rule

66. When benzene diazonium salt solution is treated with cuprous chloride, cunrous

^{roUS Cyaide 1,16 PrdUCt iS ch|}o>t>enzene, bromobenzeL or benzonytnle. These reactions are popularly known as

A.    Gatteraians reaction

B.    Schiemanns reaction

C.    Sandmeyers reaction

D.    Wurtz-Fitting reaction

⁶⁷' .^{arose gel el}ptrophoresis is very common experiment in molecular biology. This agarose is linear polysaccharide which comprises alternating units of

A.    D-galactose and 3,6-anhydrogalactose

B.    glucose and maltose

C.    P-D-fructose and a-D-fructose

D.    lactose and amylopectin

68.    Mitochondrial ATP synthesis does not require

A.    a [H⁺] gradient

B.    a membrane potential

C.    an intact inner mitochondrial membrane

D.    an electron gradient

69.    Which of the following gas evolved in Bhopal gas tragedy of 1984?

A.    Carbon monoxide and Chloroform

B.    Phosgene and Cyanamide

C.    Methyl isocyanate

D.    Ethyl isocyanate

70.    Aromatic amino acids are synthesized from which of the following precursors?

A.    Phosphoenol pyruvate, 3-phosphohydroxypyruvate

B.    Erythrose 4-phosphate, phosphoenol pyruvate

C.    Erythrose 4-phosphate, 3-phosphohydroxypyruvate

D.    Two molecules of phosphoenol pyruvate

71.    Which of the following enzyme is NOT the part glycolysis cycle?

A.    Hexokinase

B.    Enolase

C.    Acotinase

D.    Phosphoglycerate Kinase

72.    Biomolecules such as DNA, RNA, proteins and carbohydrates are made up of covalently bound constituent elements. These biomolecules are able to reversibly interact with other biomolecules by means of weaker non-covalent bonds. Which of the following statements correctly characterizes a form of non-covalent bonding?

A.    A hydrogen bond is formed between temporary dipoles

B.    An ionic bond is formed between fully charged atoms or molecules

C.    Bonds that form between non-polar groups are called van der Waals interactions.

D.    A hydrophobic bond forms between temporary dipoles

73.    The pH of a solution is the negative logarithm of the concentration of H⁴ ions. With this in mind, which of the following solution has the greatest concentration of H⁴ ions?

A.    A solution with pH=l

B.    A solution with pH=7

C.    A solution with pH=2

D.    A solution with pH=10

74.    If glucose labeled with ¹⁴C in C-l were fed to yeast carrying out the ethanol fermentation, where would the ¹⁴C label be in the products?

A.    In C-l of ethanol only

B.    In C-2 (methyl group) of ethanol only

C.    In C-2 of ethanol and CO2

D.    In CO2 only

75.    Phosphoric acid is tribasic, with p/C_as of 2.14, 6.86, and 12.4. The ionic form that predominates at pH 3.2 is

A.    H3PO4

B.    H₂P0₄

C.    HPO4²"

D.    PO4³"

76.    Compounds that generate nitrous acid (such as nitrites, nitrates, and nitrosamines) change DNA molecules by

A.    breakage of phosphodiester bonds

B.    deamination of bases

C.    depurination

D.    formation of thymine dimers

77.    During isolation and purification of genomic DNA from an organism, a proper ratio of phenol:chloroform:isoamyl alcohol is used. If proper ratio and care is not taken into consideration then chloroform may undergo oxidation in the presence of light and air and the compound formed will be

A.    dichloromethane

B.    chloretone

C.    venyl chloride

D.    phosgene

78.    A D-amino acid would interrupt an a helix made of L-amino acids. Another naturally occurring hindrance to the formation of an a helix is the presence of

A.    a negatively charged Arg residue

B.    a nonpolar residue near the carboxyl terminus

C.    a positively charged Lys residue

D.    a Pro residue

79.    For the following reaction:

[FAD] + 2 Cyt c (Fe²⁺) + 2H*- [FADH₂] + 2 Cyt c (Fe³⁺)

the electron donor is    : the reduced product is    .

A.    2Cytc (Fe²⁺);[FADH₂]    .

B.    [FAD]; [FADH₂]

C.    2 Cyt c (Fe²⁺); 2 Cyt c (Fe³⁺)

D.    [FAD]; 2 Cyt c (Fe³⁺)

80.    A gram negative coccoid Proteobacterium, found in the ocean sediments of the continental shelf of Namibia was regarded as the largest bacterium ever discovered, generally 100-300 nm wide, but sometime up to 750 jxm. This is

A.    Thiomargarita namibiensis

B.    Clostridium botulinum

C.    Treponema pallidum

D.    Haemophilus influenza

81.    Leuconostocs lack catalase and cytochromes and carry out heterolactic fermentation by converting glucose to lactate and ethanol or acetic acid by means of this pathway:

A.    Glycolytic pathway

B.    Phosphoketolase pathway

C.    Pentose phosphate pathway

D.    Gluconeogenesis

82.    The largest plant virus reported as of today

A.    Tobacco Mosaic Virus

B.    Tobacco Etch Virus

C.    Citrus Tristeza Virus

D.    Cucumber Mosaic Virus

83.    Methylotrophs are bacteria which........

A.    utilize ethanol

B.    utilize methanol

C.    produce methane

D.    produce methanol

84.    Lysine iron agar (LIA) is used to differentiate bacteria that

A.    can either deaminate or decarboxylate lysine

B.    transaminate lysine

C.    can either deaminate or transaminate lysine

D.    can either transaminate or transcarboxylate lysine

85.    For any color to be developed in the aleurone layer of com kernels, the dominant alleles at two loci plus the recessive condition at the third locus (A-R-ii) must be present. Any other genotypes will produce colorless aleurone. What phenotypic ratio of colored: colorless would be expected in the progeny from matings between parental plants of genotype AaRrli?

A.    8 colored: 56 colorless

B.    9 colored: 55 colorless

C.    58 colored: 6 colorless

D.    52 colored : 12 colorless

86.    What would be the approximate number of microorganisms present on this earth?

A.    5 x 10¹⁰

B.    5x10*

C.    5 x 10³⁰

D.    5 x 10⁴⁰

87.    Which of the following single nucleotide mutation in a mRNA codon is most likely to be a silent mutation?

A.    5CAU 3 CUU

B.    5 UUU 3 UUC

C.    5AGA3-CGA

D.    5 UAU 3-+ UAG

88. If thymine makes up 20% of the bases in a DNA molecule, what will be the percentage of cytosine in the same DNA molecule?

A.    20%

B.    30%

C.    60%

D.    80%

89. Studies based on rRNA comparison suggested that Protista was not a

following, whose contributions were highly relevant for this classification of microbes.

A.    Robert Koch

B.    Louis Pasteur

C.    Carl Woese

D.    Hargobind Khurana

90.    Enrichment culture technique and the use of selective media, which have been of

great importance in microbiology, were begun by these scientists:

A.    Robert Koch and Louis Pasteur

B.    Richrad Petri and Walther Hesse

C.    Sergei Wingradsky and Martinus Beijerinck

D.    John Tyndall and Ferdinand Kohn

91.    High frequency gene transfer of chromosomal genes can be achieved in E. coli in the following mating    

A.    Hfr X F~

B.    Hfr X Hfr

C.    F⁺ X F~

D.    F⁺ X F⁺

92.    A diploid plant has genotype AaBBccDd at 4 gene loci. In the population, there is one dominant and one recessive allele for each loci. How many different types of gametes will this individual produce?

A.    2

B.    4

C.    8

D.    16

93.    Bacterial membranes are similar to eukaryotic membranes in that many of their amphipathic lipids are phospholipids, but they usually differ from eukaryotic membranes in lacking sterols such as cholesterol; they have sterol-like molecules which might have significantly contributed to the formation of petroleum. Select the compound(s) from among the following:

A.    Ergosterols

B.    Hopanoids

C.    Phytanols

D.    Squalenes

94.    In Drosophila, the yellow (body color) gene is located 2 cM away from the white (eye color) gene locus. In female heterozygous for both recessive gene loci, what fraction of the gametes would be expected to have undergone a crossover between these genes?

A.    0.02

B.    0.2

C.    0.01

D.    0.1

95.    Cyanobacteria, myxobacteria, cytophagas, and some mycoplasmas have a different type of motility with no visible external structures associated with this kind of motility that is popularly known as

*

A.    Chemotactic motility

B.    Gliding motility

C.    Cork screw motility

D.    Phototactic motility

96.    Identify the causative agent from Column B (1,2, 3,4, 5 and 6) for the following diseases from Column A (L, M, N and O)

Column A    Column B

L. Toxic Shock Syndrome    1. Clostridium perjringens

M. Gas gangrene    2. Mycobacterium leprae

N. Kuru Leprosy    3. Prions

O. Sleeping sickness    4. Trypanosoma gambiense

5.    Leishmania donovani

6.    Staphylococcus aureus    -

A.    L-6; M-l; N-3; 0-4

B.    L-6; M-l; N-3; 0-5

C.    L-6; M-3; N-2; 0-5

D.    L-3; M-l; N-2; 0-5

97. Some amino acids have ionizable side chains. Which amino acid would have this titration curve ?

A.    Tyrosine

B.    Aspartate

C.    Cysteine

D.    Glycine

98.    Sex-linked recessive alleles are usually carried on:

A.    The part of the X chromosome homologous with Y chromosome

B.    The part of the X chromosome non-homologous with Y chromosome

C.    The part of the Y chromosome homologous with X chromosome

D.    Autosomes but expressed in hemizygous or homozygous state

99.    Identify the following antibiotics from Column A (L, M, N and O) and their mode of action from Column B (1,2,3,4 and 5)

Column A    Column B

1.    Antibacterial

2.    Antimalarial

3.    Antiviral

4.    Antifungus

5.    Anticancer and antitumor

A.    L-5;M-4;N-l;0-2

B.    L-l; M-4; N-3; 0-2

C.    L-5; M-3; N-l; 0-4

D.    L-l; M-4; N-5; 0-2

100. Gram positive bacteria have

A.    thick, homogeneous layers of peptidoglycan and teichoic acids .

B.    thin, heterogeneous layers of peptidoglycan, teichoic acids and lipopolysaccharides

C.    thin, peptidoglycan layer surrounded by a complex outer membrane containing lipopolysaccharides

D.    thick, homogeneous layers of peptidoglycan and lipopolysaccharides