Kerala University 2009 B.Tech Biotechnology Seventh Semester , (2003 Scheme) Branch : 03-704 : Downstream Processing (B) - Question Paper
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Reg. No. :.....................................
Name :..........................................
Seventh Semester B.Tech. Degree Examination, June 2009
(2003 Scheme)
Branch : BIOTECHNOLOGY 03-704 : Downstream Processing (B)
Time : 3 Hours Max. Marks : 100
PART - A
Answer all questions in Part A.
1. What are the limitations of centrifugation in the separation of biomolecules ?
2. Explain cross-flow filtration.
3. How does a cost-cutting strategy influence the quality of the product ?
4. Explain the use of SDS in downstream operations.
5. Describe a typical ultrafiltration unit with a neat diagram.
6. What do you understand by the term distribution ratio in the context of enrichment operations ?
7. What are the disadvantages of integrated bioprocessing ?
8. Explain the technique of immunosorption.
9. What is the significance of a break-through curve ?
10. Give examples of typical membranes employed in protein purification.
(10x4=40 Marks)
11. Enumerate the general procedures employed for the isolation of a fermentation product. Explain with a typical example from the pharmaceutical industry. 20
12. What are the primary factors affecting separation ? How are they influenced by particle size ? 20
Module - II
13. Explain
14. Explain
i) Fractional precipitation. 10
ii) Camp Number. 10
Module - III
15. Explain the basic phenomenas underlying four different classes of chromatographic separations. 20
16. Describe a product recovery train of a typical operation by using a flow diagram. 20
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