Biju Patnaik University of Technology 2008 B.E Biomedical Engineering Biochemistry - Question Paper
4th Semester exam – 2008
BIOCHEMISTRY
Total number of printed pages -6 B. Tech
CPBM 8201Fourth Semester Examination - 2008 BIOCHEMISTRY
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Full Marks-70
Time: 3 Hours
Answer Question No. 1 which is compulsory and any five from the rest.
The figures in the right-hand margin indicate marks.
1. Answer the following questions : 2 x 10
(a) What forces hold protein subunits in the secondary and quaternary structure of proteins ?
(b) What is the fate of radio active label C14 labeled at C-2 positions of Glucose-6-phosphate added to a cell extract containing all the enzymes and cofactors of TCA cycle ?
(c) Predict the migration direction (Anodal, Cathodal or Stationary) during separation of following peptide at pH 6.0 : Lys-Trp-Cys-Gly-Ala-GIu.
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(d) Justify the role of heamoglobin as buffering agent in blood.
(e) Define isoelectric point. Calculate the isoelectric point of Cysteine if pKa (a-COOH), pKa(a-NH3+) and pKa R(side chain) values are 1.7,10.8 and 8.3 respectively.
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(f) Why do differences in melting point exist between fatty acids containing same number of carbon atoms ?
(g) Differentiate between peptide bond and phosphodiester bond with neat and labeled diagram.
CPBM 8201 2 Contd.
(h) What do you mean by redox potential ? Calculate redox potential of half reaction-1/2 O1 + 2H+ + 2 e" -> H20 at temperature 25 C and pH 7.0?
(i) The normal concentration of glucose-6-phosphate (G-6-P) and fructose-6-phos-phate (F-6-P) in human erythrocytes are 1 x 10_5M and 1 x 10-6M, respectively. If the standard free energy change (AG) for the reaction G-6-P to F-6-P is 0.4 Kcal/ mol. Calculate the free energy change (AG) for the conversion of G-6-P to F-6-P.
(j) What is the significance of hexose monophosphate (HMP) shunt in RBC from clinical point of view?
3. (a) Explain the methods used for isolation, purification and quantification of protein molecules. 5
(b) Explain the diagnostic assay for Renal function assessment. 5
4. Explain the various level of organization of protein structure with emphasis on various bonds and chemical interaction on protein function.
10
5. (a) One molecule of Lactic acid
(CH3CH0HC02H) is oxidized completely to C02 and H20. Calculate the number of energy rich phosphate bonds that should be produced when each compound is oxidized, accounting for the consumption of energy rich phosphate if any. 4
(b) Briefly explain the carriers of Electron transport system involved in the oxidative
CPBM 8201 4 Contd.
phosphorylation of NADH2 with a note on its ATP generation. 6
6. Define liver dysfunction. Briefly explain the various kinds of liverfunction tests based upon different functions of liver in human ? 10
7. Write short notes: 2.5 x 4
(a) a- Oxidation
(b) Gluconeogenesis
(c) Acid-base balance concept
(d) Z-DNA.
8. (a) Define Isotachophoresisis. What is its
significance ? 3
(b) The absorptivity ofthe copper (Il)-protein complex which is formed in the biuret reaction is 0.05 cm2mg-1 at 545 nm. Calculate the protein concentration if the absorbance is 0.42. 3
(c) Define radioactive decay. What are the units of measurement of radioactive decay. An experimental sample of 3H on filter paper in scintillation fluid gave count rate of 1450 cpm in liquid scintillation counter. The filter was removed and 5064 dpm added to it. On recounting, the filter gave a reading of 2878 cpm. What is the dpm of the experimental sample. 4
CPBM 8201 6 -C
What is fluid mosaic model of biological membrane ? Describe various transport systems that move molecules across the cell membrane with note on Na-K pump. 10
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