Vinayaka Missions University 2007 B.E Industrial Bio-Technolgy GENETIC ENGINEERING - Question Paper

What is meant by unique Restriction sites?
elaborate Lambda vectors?
provide an example for l Phage vector.
provide the selectable markers for Lambda vectors.
provide the selectable markers used in improved l Phage vectors.
discuss about M13 Phage vector.
elaborate Cosmid vectors.
elaborate Retro viral vectors?
Comment on Baculovirus vectors.
discuss the role of Plasmid as vectors.
Write notes on Bacterial Artificial Chromosomes.
Write notes on Yeast Artificial Chromosomes.
PART B
discuss the properties of pBR322.
provide the properties of pUC Vectors.
provide the properties of Lambda Vectors.
provide the advantages of Phage vectors over Plasmid vectors.
Write an detailed notes on Cosmid Vectors.
Brief account on Artificial chromosomes.
elaborate Bacterial artificial chromosomes (BACs)?
elaborate Yeast artificial chromosomes (YACs)?
discuss about the cloning in Bacteria other than E. coli.
discuss in detail the cloning vectors used for Gram negative bacteria.
discuss in detail the cloning vectors used for Gram positive bacteria.
discuss in detail Prokaryotic and Eukaryotic expression vectors.
Write about Bacculoviral expression vectors.
discuss Retroviral expression vectors.
Unit III
PART A
elaborate the basic steps involved in constructing recombinant DNA?
describe Competency.
What is the role of calcium chloride in preparing Competent cells.
Write short notes on electroporation.
What is Transfection?
describe Transformation?
What is meant by somatic cell fusion?
describe ‘Protoplast’.
What is meant by differential screening?
Write a note on YES Vectors.
Account on SV Vectors.
What is the function of RI Plasmid?
Write a note on promoters used in Yeast expression system.
Write a brief note on Ti Plasmid.
Write short notes on T DNA.
elaborate Co-integration and Binary Vectors?
Comment briefly on various promoters used for expression in plants.
provide an account on Gene transfer.
What is shot gun method of Gene transfer?
What is Nuclear injection method?
PART B
describe Competency. define the protocol for the preparation of Competent cells.
elaborate the various methods of Gene Transformation?
elaborate the various methods of Gene Transfection?
Write an essay on Expression system using Escherichia coli
Write notes on Expression system using Streptomyces.
provide a detailed account on Expression system using Yeast.
Write an essay on Agrobacterium mediated transformation.
elaborate Ti Plasmids? discuss with a neat tagged diagram.
Write a short note on the following:
a. Short gun method of gene transfer
b. Nuclear injection method
define different methods of Gene transfer in plants.
Unit iv
PART A
describe Genomic library.
What is cDNA library?
Write on In situ Hybridization.
elaborate the various methods for comparing Transcriptomes?
discuss briefly on differential screening and differential display.
What is meant by reporter genes?
discuss briefly about Gel retardation assays.
Write briefly on Yeast 1 hybrid systems.
elaborate the various methods for Translational analysis?
What is Chromosome walking?
Write short notes on Chromosome jumping.
Write notes on any 2 DNA Probe.
provide the applications of DNA probes.
provide the applications of molecular markers in Recombinant DNA technology.
elaborate Minisatellites and Microsatellites?
elaborate the steps involved in Restriction mapping?
elaborate the steps involved in Gene Targeting?
What is Transcript mapping?
provide an account on Gene targeting.
describe Transposons.
PART B
Write a detailed account on the construction of Genomic library.
Briefly define the methods used in screening and selection of Genomic library.
discuss how cDNA Libraries are constructed?
discuss the different methods used in the analysis of Gene expression.
Write a short note on the subsequent.
a. Chromosome walking
b. Chromosome jumping
Write an essay on Restriction mapping and its applications.
provide a detailed account on the applications of Gene targeting.
define briefly about the different molecular markers used in Recombinant DNA technology.
What is Transcript mapping? define the different methods used for Transcript mapping
elaborate Transposons? discuss Transposon tagging with examples.
UNIT V
PART A
describe Mutagenesis?
elaborate ‘Mutants’?
Write on Cassette Mutagenesis.
Write an account on Deletion mutagenesis.
discuss briefly Oligonucleotide derived mutagenesis
Write a note on Site directed mutagenesis.
What is DNA fingerprinting?
provide the basis of DNA Fingerprinting?
Expand RFLP and mention its applications.
discuss Biodegradable plastics.
Write about any 2 Recombinant proteins used in diagnostic purpose.
What is Animal pharming?
What is Microinjection?
List out a few applications of Transgenic plants and animals.
Mention any 2 Therapeutic vaccines produced by rDNA technology.
What is antisense RNA?
provide any 2 applications of Antisense RNA technique.
What is meant by Biosafety?
Comment on various Biosafety Levels.
Mention any 2 safety guide lines for rDNA technology
PART B
explain on Site-directed mutagenesis and its applications.
Write an essay on the applications of rDNA technology in producing recombinant proteins.
define how Biodegradable plastics can be produced by rDNA technology
Write a detailed account on the applications of rDNA technology in Pathogenesis and Diagnostics.
provide a detailed account on the applications of rDNA technology for producing therapeutic vaccine.
Briefly define the methods developed for the production of Transgenic plants and animals.
define Antisense - RNA technique and its applications.
elaborate the safety lines for rDNA techniques?
provide a detailed account on the guidelines for disposal of Biowastes.

Earning:   Approval pending.