Vinayaka Missions University 2007 B.E Industrial Bio-Technolgy GENETIC ENGINEERING - Question Paper

GENETIC ENGINEERING
PART A
elaborate called as Sticky or Cohesive ends?
discuss the kind II Restriction enzymes.
provide the function of DNA ligase.
provide the use of linkers in Recombinant DNA technology.
define briefly about any 2 enzymes used in non – radioactive labeling of Nucleic acid.
discuss labeling by Nick translation.
provide the function of Taq DNA polymerase.
elaborate the components needed for PCR?
provide any 2 applications of PCR.
What is meant by RT – PCR?
Write a note on Application of RT – PCR.
discuss Ligase Chain Reaction?
provide the application of Ligase chain reaction.
elaborate the chemical agents used to replace DNA for Maxam and Gilbert method?
provide the function of Piperdine in Maxam and Gilbert DNA sequencing method.
discuss Ligase Chain Reaction.
describe Heteroduplexing.
What is meant by Northern blot?
Compare the properties of Nitrocellulose membrane with nylon membrane.
What is meant by Filter hybridization?
Write briefly about the hybridization probes used in Nucleic acid labeling.
elaborate the steps involved in gel pretreatment during Hybridization?
provide any 2 blocking agents used in Hybridization.
Write short notes on Southern blotting.
provide any 2 applications of Blotting techniques.
PART B
elaborate Restriction enzymes? Write about their Nomenclature and about the various classes of Restriction enzymes.
discuss the various methods of Cutting DNA molecules.
elaborate the various methods of joining 2 DNA molecules? discuss.
discuss the various methods of DNA labeling.
discuss the various Radioactive labeling methods.
Write in detail about the various Non-Radioactive tags.
Compare Radioactive labeling and Non – Radioactive labeling methods available for Nucleic acid labeling.
discuss Polymerase Chain Reaction and its application.
Write an account on RAPD and its significance.
define Maxam and Gilbert method of DNA sequencing.
provide various kind of Nucleic acid hybridization and its applications.
discuss the principle behind Nucleic acid hybridization.
define Southern hybridization and its application.
discuss Western Blotting and its applications.
UNIT II
PART A
elaborate the various methods involved in Plasmid isolation?
provide any 2 naturally occurring Plasmid.
elaborate Cloning vectors and Expression vectors?
How do you convert low copy plasmids into high copy plasmids?
What is meant by Plasmid incompatibility?
elaborate Shuttle vectors?
discuss R1 Plasmid.
provide the properties of Col E1 Plasmids.
Write short notes on pSC 101 Vectors.
elaborate the advantages and disadvantages of using Plasmids as vector DNA?
Write notes on pBR 322 Vector.
elaborate pUC vectors?
What is meant by insertional inactivation?

Earning:   Approval pending.