Mahatma Gandhi University (MGU) 2012-2nd Sem M.Sc Microbiology , Kottayam - (MODEL ) MB2PG07- Molecular Biology and Genetic Engineering - Question Paper

Mahatma Gandhi University, Kottayam
Second Semester M.Sc.Microbiology exam - 2012
(MODEL ques. PAPER)
MB2PG07- Molecular Biology and Genetic Engineering
Time : three Hrs Weightage: 30
I. Write Notes on Any 5 (Wt: one each)
1. Restriction endonucleases
2. Gene chip
3. palindromic sequences
4. YAC
5. C-value paradox
6. Ribozymes
7. Okazaki fragments
8. Topoisomerase
II Write Short Essay on Any 5 (Wt: two each)

9. Post transcriptional modification of eukaryotic mRNA
10. RNA polymerases in prokaryotes and enkaryotes,
11. Wobble hypothesis
12. DNA modifying enzymes.
13. Transgenic plants
14. Gene knockout technique
15. Expression vectors
16. Difference ranging from Southern and Northern blotting Techniques
III ans Any 3 in Detail (Wt: five each)
17. explain in detail lac operon system and its regulatory components.
18. provide a brief definition of a gene library. What is the essential difference ranging from a
genomic library and a cDNA library? List the major advantages/limitations on the use of
each.
19. Write in detail about molecular markers and their importance.
20. Write about primer designing in PCR. Which DNA polymerase is used in PCR?
21. discuss the various stages in transcription. Add a note on regulation of transcription.
22. define in detail the role of enzymes in DNA replication.

Earning:   Approval pending.